Gastric adenocarcinoma is the second leading cause of cancer-related death in the world. H. pylori is the strongest known risk factor for this malignancy, yet only a fraction of infected persons ever develop cancer. One H. pylori determinant that augments cancer risk is the cag pathogenicity island, and several cag genes encode components of a type IV bacterial secretion system which functions to export proteins (e.g. CagA) into host epithelial cells. A host effector that may influence carcinogenesis is B-catenin, a downstream component of the Wnt pathway. When Wnt signaling is inactive, B-catenin is constitutively phosphorylated and degraded; binding of Wnt to its receptor inhibits (3-catenin phosphorylation, leading to its nuclear accumulation and the transcriptional activation of genes that influence carcinogenesis. Nuclear accumulation of (3-catenin is increased in gastric adenoma and dysplasia specimens, histologic stages that precede gastric adenocarcinoma. Our preliminary studies now demonstrate that a rodent-adapted H. pylori cag+ strain (7.13) rapidly induces gastric cancer in hypergastrinemic (INS-GAS) mice by 24 weeks and in Mongolian gerbils by 4 weeks. H. pylori strain 7.13 also induces nuclear translocation of B-catenin and activates a B-catenin-responsive reporter in vitro, indicating that B-catenin is functionally responsive to this prototype strain. B-catenin activation by H. pylori is dependent .upon translocation of CagA into epithelial cells, and nuclear accumulation of B-catenin is increased in gastric epithelium harvested from cag+-infected persons, compared to subjects carrying cag' strains or uninfected persons. Our hypothesis is that H. pylori cag* strains selectively activate host signaling pathways, such as those mediated by B-catenin. thereby regulating cellular responses that contribute to the augmentation in carcinogenic risk associated with these strains. Thus, our specific aims are: 1. To define the effects of H. pyloriconstituents on activation of B-catenin in vitro and in vivo. 2. To define the host signaling pathways that regulate H. py/ori-induced B-catenin activation. 3. To define differences in epithelial molecular responses to carcinogenic H. pylori versus mutant strains using a transgenic murine model of gastric cancer.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK073902-03
Application #
7332235
Study Section
Special Emphasis Panel (ZRG1-DIG-C (02))
Program Officer
Hamilton, Frank A
Project Start
2006-04-01
Project End
2010-12-31
Budget Start
2008-01-01
Budget End
2008-12-31
Support Year
3
Fiscal Year
2008
Total Cost
$290,565
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Mera, Robertino M; Bravo, Luis E; Camargo, M Constanza et al. (2018) Dynamics of Helicobacter pylori infection as a determinant of progression of gastric precancerous lesions: 16-year follow-up of an eradication trial. Gut 67:1239-1246
Varga, Matthew Gordon; Peek, Richard M (2017) DNA Transfer and Toll-like Receptor Modulation by Helicobacter pylori. Curr Top Microbiol Immunol 400:169-193
Noto, Jennifer M; Peek Jr, Richard M (2017) Helicobacter pylori Makes a Molecular Incision to Gain Epithelial Entry. Cell Host Microbe 22:434-436
Noto, Jennifer M; Peek Jr, Richard M (2015) Micronutrients: A double-edged sword in microbial-induced gastric carcinogenesis. Trends Cancer 1:136-144
Wroblewski, Lydia E; Piazuelo, M Blanca; Chaturvedi, Rupesh et al. (2015) Helicobacter pylori targets cancer-associated apical-junctional constituents in gastroids and gastric epithelial cells. Gut 64:720-30
Wei, Jinxiong; Noto, Jennifer M; Zaika, Elena et al. (2015) Bacterial CagA protein induces degradation of p53 protein in a p14ARF-dependent manner. Gut 64:1040-8
Noto, Jennifer M; Peek Jr, Richard M (2012) Genetic manipulation of a naturally competent bacterium, Helicobacter pylori. Methods Mol Biol 921:51-9
Noto, Jennifer M; Peek Jr, Richard M (2012) The Helicobacter pylori cag Pathogenicity Island. Methods Mol Biol 921:41-50
Sicinschi, L A; Correa, P; Peek, R M et al. (2010) CagA C-terminal variations in Helicobacter pylori strains from Colombian patients with gastric precancerous lesions. Clin Microbiol Infect 16:369-78
Israel, Dawn A; Peek, Richard M (2010) Surreptitious manipulation of the human host by Helicobacter pylori. Gut Microbes 1:119-127

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