Mammals regulate fat mass, so that in weight-stable individuals increases or reductions in adipose tissue activate responses that favor return to one's previous weight: A reduction in fat mass activates a system that increases food intake and reduces energy expenditure, and conversely, overfeeding and rapid adipose tissue expansion reduces food intake and increases energy expenditure. With the identification of leptin nearly two decades ago the central circuit that defends against reductions in body fat was revealed. Since then, the cellular and molecular pathways that leptin regulates in defense of body fat have been intensively and fruitfully studied. However, the systems that defend against rapid expansion of fat mass have been less well studied and characterized. Indeed, the key components remain largely obscure. During an effort to characterize the role that immune cells play in metabolism we uncovered a system that we believe limits fat mass expansion. The central component of this system is the activation of pro- apoptotic signaling in adipose tissue macrophages (ATMs). As adipose tissue expands, ATMs increase in number and accumulate large amounts of lipid, increasing cellular stress and their susceptibility to apoptosis. In studyig strains of mice that vary in their susceptibility to high fat diet-induced obesity, we found that te size of the circulating natural killer (NK) cell population predicts adipose tissue NK cell content the size of the CD11c+ ATM population, and resistance to weight gain. Animals with the highest concentration of NK cells in their circulation and adipose tissue have fewer CD11c+ ATMs and are resistant to the development of obesity. Classically, NK cells recognize and induce apoptosis in malignant, virally infected and certain populations of stressed cells. We found that obesity increases the expression of an NK cell-recognized stress signal (RAET1e) in adipose tissue and specifically upregulates its expression in lipid-laden CD11c+ ATMs that are reduced in strains with high NK cells. These data suggested that NK cell targeted apoptosis of CD11c+ ATMs could limit fat mass expansion. Indeed, direct activation of apoptosis of CD11c+ ATMs in obese, but not in lean mice, reduces food intake and induces weight loss, without evidence of an inflammatory reaction or lipodystrophy. We propose to test (1) whether genetically decreasing or increasing NK cell targeted apoptosis of ATMs will predictable reduce or increase fat mass in high fat fed mice (2) whether weight loss induced by ATM apoptosis increases energy expenditure and requires leptin, and (3) whether overfeeding mice activates NK cell targeted apoptosis of ATMs to limit adipose tissue expansion. Achieving the goals of this application will identify potential therapeutic strategies to reduce or limit adipose tissue mass b targeted manipulation of adipose tissue immune cells.

Public Health Relevance

Obesity results from the accumulation of excess fat tissue and is a leading cause of type 2 diabetes, non- alcoholic fatty liver disease and dyslipidemia. The body has systems that regulate the total amount of fat and while much has recently been learned about the response to reduced fat mass less is now about how the body limits fat mass. Understanding how the body limits fat tissue expansion will identify potential therapeutic strategies to combat obesity and obesity-associated diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
1R01DK101942-01
Application #
8672138
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Haft, Carol R
Project Start
2014-04-14
Project End
2018-02-28
Budget Start
2014-04-14
Budget End
2015-02-28
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Columbia University (N.Y.)
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10032
Ravussin, Yann; Edwin, Ethan; Gallop, Molly et al. (2018) Evidence for a Non-leptin System that Defends against Weight Gain in Overfeeding. Cell Metab 28:289-299.e5
Chang, Hye Rim; Kim, Hae Jin; Xu, Xiaoyuan et al. (2016) Macrophage and adipocyte IGF1 maintain adipose tissue homeostasis during metabolic stresses. Obesity (Silver Spring) 24:172-83
Grijalva, Ambar; Xu, Xiaoyuan; Ferrante Jr, Anthony W (2016) Autophagy Is Dispensable for Macrophage-Mediated Lipid Homeostasis in Adipose Tissue. Diabetes 65:967-80
Edwin, Ethan A; Ferrante Jr, Anthony W (2015) Keeping Off the Weight with DCs. Immunity 43:624-6
Subramanian, Manikandan; Ozcan, Lale; Ghorpade, Devram Sampat et al. (2015) Suppression of Adaptive Immune Cell Activation Does Not Alter Innate Immune Adipose Inflammation or Insulin Resistance in Obesity. PLoS One 10:e0135842
Ravussin, Yann (2015) Temperature matters with rodent metabolic studies. Obesity (Silver Spring) 23:1330
Ravussin, Yann; Leibel, Rudolph L; Ferrante Jr, Anthony W (2014) A missing link in body weight homeostasis: the catabolic signal of the overfed state. Cell Metab 20:565-72
Song, Fei; Hurtado del Pozo, Carmen; Rosario, Rosa et al. (2014) RAGE regulates the metabolic and inflammatory response to high-fat feeding in mice. Diabetes 63:1948-65