In vivo fluorescence imaging, allowing the visualization of physiological processes in an intact living organism, has been an indispensable preclinical tool for fundamental understandings of disease pathology and development. More recently, it has also been translated into clinical trials to improve surgery outcomes and lower health-care costs. However, delivery of in vivo fluorescence imaging into preclinical kidney research and surgery remains highly challenging because of undesired in vivo behaviors such as rapid kidney clearance and high accumulation in background tissues of current NIR emitting contrast agents. As a result, in vivo kidney imaging remains heavily relied on high-cost radiological imaging techniques such as positron emission tomography (PET) and single-photon emission computed tomography (SPECT), which not only limits fundamental kidney research but also makes it challenging for surgeons to conduct imaging-guided kidney surgery in the future. The objective of this application to address these critical challenges by developing a class of renal clearable near infrared (NIR) emitting nanoprobes with high kidney-targeting specificity, tunable kidney retention time and minimum accumulation in normal background tissues and reticuloendothelial system (RES) organs, so that both noninvasive and invasive kidney imaging can be achieved at high contrast with desired detection time window.

Public Health Relevance

One in 10 adults in the United States suffers from a variety of kidney diseases; thus developing low- cost and high-sensitivity imaging techniques that can advance our fundamental understandings of underlying mechanisms of kidney diseases and drug development will be highly desired but remains highly challenging. We aim to develop a new class of near infrared (NIR)-emitting nanoparticles with desired in vivo behaviors that can significantly enhance kidney-imaging contrast more than one order of magnitude over current fluorescent contrast agents while allow imaging-time window to be optimized for different imaging requirements. Success of the proposed work will enable in vivo fluorescence imaging to serve as a practical tool to promote preclinical kidney research and catalyze new paradigm shifts in imaging guided kidney surgery. 1

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
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Biomaterials and Biointerfaces Study Section (BMBI)
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Gossett, Daniel Robert
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University of Texas-Dallas
Schools of Arts and Sciences
United States
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Jia, Jie-Min; Peng, Chuanqi; Wang, Yihui et al. (2018) Control of occlusion of middle cerebral artery in perinatal and neonatal mice with magnetic force. Mol Brain 11:47
Xu, Jing; Yu, Mengxiao; Peng, Chuanqi et al. (2018) Dose Dependencies and Biocompatibility of Renal Clearable Gold Nanoparticles: From Mice to Non-human Primates. Angew Chem Int Ed Engl 57:266-271
Huang, Yingyu; Fuksman, Lirit; Zheng, Jie (2018) Luminescence mechanisms of ultrasmall gold nanoparticles. Dalton Trans 47:6267-6273
Xu, Jing; Peng, Chuanqi; Yu, Mengxiao et al. (2017) Renal clearable noble metal nanoparticles: photoluminescence, elimination, and biomedical applications. Wiley Interdiscip Rev Nanomed Nanobiotechnol 9:
Du, Bujie; Jiang, Xingya; Das, Anindita et al. (2017) Glomerular barrier behaves as an atomically precise bandpass filter in a sub-nanometre regime. Nat Nanotechnol 12:1096-1102
Yu, Mengxiao; Zhou, Chen; Liu, Li et al. (2017) Interactions of Renal-Clearable Gold Nanoparticles with Tumor Microenvironments: Vasculature and Acidity Effects. Angew Chem Int Ed Engl 56:4314-4319
Xu, Jing; Yu, Mengxiao; Carter, Phoebe et al. (2017) In?Vivo X-ray Imaging of Transport of Renal Clearable Gold Nanoparticles in the Kidneys. Angew Chem Int Ed Engl 56:13356-13360
Peng, Chuanqi; Gao, Xiaofei; Xu, Jing et al. (2017) Targeting orthotopic gliomas with renal-clearable luminescent gold nanoparticles. Nano Res 10:1366-1376
Liu, Jinbin; Duchesne, Paul N; Yu, Mengxiao et al. (2016) Luminescent Gold Nanoparticles with Size-Independent Emission. Angew Chem Int Ed Engl 55:8894-8
Sun, Shasha; Ning, Xuhui; Zhang, Greg et al. (2016) Dimerization of Organic Dyes on Luminescent Gold Nanoparticles for Ratiometric pH Sensing. Angew Chem Int Ed Engl 55:2421-4

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