? In this proposal, novel bioadhesives termed """"""""interracial biomaterials"""""""" are described to improve implanttissue integration. These interfacial biomaterials can adhere specific cell types to a synthetic or natural substrate while mediating specific cell functions. These functional interfacial biomaterials address a major thrust of modern surgical medicine: """"""""smart"""""""" biomaterials that promote a cellular/tissue response through discrete interactions at the molecular level. Phage display technology will be used to identify peptide sequences that bind selectively to one target such as a polymer (e.g., collage type I, poly(glycolic acid)) or a cell type (e.g., endothelial cells, smooth muscle cells). The adhesive strength and selectively of the peptide to a given target will then be determined. Next, these peptides will be synthesized using solid-phase peptide synthesis techniques and then assembled to create an interfacial biomaterial (IFBM). These tFBMs will be applied at the interface to secure biologics to a synthetic surface. Although numerous biomedical applications are envisioned, this proposal will focus on understanding the biological, chemical, and engineering principles that govern the adhesion and function of endothelial cells on IFBM coated engineered vascular grafts. Specific IFBMs will be developed to improve the in vivo performance of tissue engineered vascular grafts by reducing or eliminating graft thromogenicity. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB000501-03
Application #
6750062
Study Section
Surgery and Bioengineering Study Section (SB)
Program Officer
Lee, Albert
Project Start
2003-07-01
Project End
2007-06-30
Budget Start
2004-07-01
Budget End
2005-06-30
Support Year
3
Fiscal Year
2004
Total Cost
$319,903
Indirect Cost
Name
Boston University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
049435266
City
Boston
State
MA
Country
United States
Zip Code
02215
Meyers, Steven R; Grinstaff, Mark W (2012) Biocompatible and bioactive surface modifications for prolonged in vivo efficacy. Chem Rev 112:1615-32
Meyers, Steven R; Kenan, Daniel J; Khoo, Xiaojuan et al. (2011) Bioactive stent surface coating that promotes endothelialization while preventing platelet adhesion. Biomacromolecules 12:533-9
Huang, Xin; Zauscher, Stefan; Klitzman, Bruce et al. (2010) Peptide interfacial biomaterials improve endothelial cell adhesion and spreading on synthetic polyglycolic acid materials. Ann Biomed Eng 38:1965-76
Meyers, Steven R; Khoo, Xiaojuan; Huang, Xin et al. (2009) The development of peptide-based interfacial biomaterials for generating biological functionality on the surface of bioinert materials. Biomaterials 30:277-86
Meyers, Steven R; Kenan, Daniel J; Grinstaff, Mark W (2008) Enzymatic release of a surface-adsorbed RGD therapeutic from a cleavable peptide anchor. ChemMedChem 3:1645-8
Kenan, Daniel J; Walsh, Elisabeth B; Meyers, Steven R et al. (2006) Peptide-PEG amphiphiles as cytophobic coatings for mammalian and bacterial cells. Chem Biol 13:695-700