) This proposal addresses three important problem in biomedical science via the development of simple and rapid assays for biomolecules. The first goal involves the development of a room temperature color assay for fructose, a ubiquitous, rapidly metabolized sugar. The proposedassay is designed to be fructose-selective in the presence of a large excess of glucose. Fructose determination in plasma is currently hampered due to the large excess of glucose. The second goal involves creatinga mild assay for sialic acid, an important cell surface antigen, to address the problem of current assay inaccuracy based on hydrolytic procedures and/or relatively non-specific and harsh methods. The third goal entails the implementation of a new automated color detection system for oligosaccharides that does not require prior destructive hydrolysis or chemical labeling with a chromophore. Currently no detection technique exists for neutral oligosaccharides that is effective for the visual detection of oligosaccharides beyond three repeat units.
| Chu, Yu-Hsuan; Escobedo, Jorge O; Jiang, Meiyan et al. (2016) Rhodamine analogues for molecular ruler applications. Dyes Pigm 126:46-53 |
| Yang, Xiao-Feng; Huang, Qian; Zhong, Yaogang et al. (2014) A dual emission fluorescent probe enables simultaneous detection of glutathione and cysteine/homocysteine. Chem Sci 5:2177-2183 |
| Hakuna, Lovemore; Escobedo, Jorge O; Lowry, Mark et al. (2014) A photochemical method for determining plasma homocysteine with limited sample processing. Chem Commun (Camb) 50:3071-3 |
| Escobedo, Jorge O; Chu, Yu-Hsuan; Wang, Qi et al. (2012) Live cell imaging of a fluorescent gentamicin conjugate. Nat Prod Commun 7:317-20 |
| Morakinyo, Moshood K; Chipinda, Itai; Hettick, Justin et al. (2012) Detailed mechanistic investigation into the S-nitrosation of cysteamine. Can J Chem 9:724-738 |
| Sibrian-Vazquez, Martha; Escobedo, Jorge O; Lowry, Mark et al. (2012) Progress toward red and near-infrared (NIR) emitting saccharide sensors. Pure Appl Chem 84:2443-2456 |
| Guo, Yixing; Yang, Xiaofeng; Hakuna, Lovemore et al. (2012) A fast response highly selective probe for the detection of glutathione in human blood plasma. Sensors (Basel) 12:5940-50 |
| Sibrian-Vazquez, Martha; Escobedo, Jorge O; Lowry, Mark et al. (2012) Field effects induce bathochromic shifts in xanthene dyes. J Am Chem Soc 134:10502-8 |
| Yang, Xiaofeng; Guo, Yixing; Strongin, Robert M (2012) A seminaphthofluorescein-based fluorescent chemodosimeter for the highly selective detection of cysteine. Org Biomol Chem 10:2739-41 |
| Ye, Yanping; Yaeger, Daniel; Owen, Laura J et al. (2012) Designing calcium release channel inhibitors with enhanced electron donor properties: stabilizing the closed state of ryanodine receptor type 1. Mol Pharmacol 81:53-62 |
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