The broad, long-term objective of this project is the development of a non-invasive optical technique for imaging blood flow in human skin with high spatial resolution. Optical Doppler Tomography (ODT) combines Doppler velocimetry with optical coherence tomography (OCT) to measure blood flow velocity at discrete user-specified locations in highly scattering biological tissues. The exceptionally high resolution of ODT allows noninvasive imaging of both in vivo blood microcirculation and tissue structures surrounding the vessel. Our central hypothesis in this research proposal is that ODT provides a practical methodology for characterization of port wine stain (PWS) birthmarks, prior to institution of laser therapy. To this end, we propose four specific aims: (1) an ODT system based on spectral interferometry will be designed and constructed; (2) an imaging construction algorithm will be developed; (3) a series of experiments to image in vivo blood flow will be carried out using the high speed ODT system to confirm image accuracy and fidelity; and (4) demonstrate in clinical studies how ODT can assist in the clinical management of PWS patients receiving laser therapy. In preliminary studies conducted on in vitro turbid samples, in vivo model vasculatures and in situ PWS human skin, the feasibility and potential application of ODT to characterize and image blood flow with high spatial resolution at discrete user-specified locations in highly scattering biological tissues such as human skin has been demonstrated. The objective of the studies proposed herein is to extend these initial findings and is expected to lead to the development of a new technology for the clinical management of PWS patients.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB002495-04
Application #
7104887
Study Section
Special Emphasis Panel (ZRG1-SRB (53))
Program Officer
Haller, John W
Project Start
2003-09-30
Project End
2008-07-31
Budget Start
2006-08-01
Budget End
2007-07-31
Support Year
4
Fiscal Year
2006
Total Cost
$297,833
Indirect Cost
Name
University of California Irvine
Department
Surgery
Type
Schools of Medicine
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
Bae, Youngwoo; Son, Taeyoon; Stuart Nelson, J et al. (2011) Dermatological feasibility of multimodal facial color imaging modality for cross-evaluation of facial actinic keratosis. Skin Res Technol 17:4-10
Nelson, J Stuart; Jia, Wangcun; Phung, Thuy L et al. (2011) Observations on enhanced port wine stain blanching induced by combined pulsed dye laser and rapamycin administration. Lasers Surg Med 43:939-42
Yoon, Jinhee; Park, Donghee; Son, Taeyoon et al. (2010) A physical method to enhance transdermal delivery of a tissue optical clearing agent: combination of microneedling and sonophoresis. Lasers Surg Med 42:412-7
Loewe, Robert; Oble, Darryl A; Valero, Teresa et al. (2010) Stem cell marker upregulation in normal cutaneous vessels following pulsed-dye laser exposure and its abrogation by concurrent rapamycin administration: implications for treatment of port-wine stain birthmarks. J Cutan Pathol 37 Suppl 1:76-82
Jia, Wangcun; Sun, Victor; Tran, Nadia et al. (2010) Long-term blood vessel removal with combined laser and topical rapamycin antiangiogenic therapy: implications for effective port wine stain treatment. Lasers Surg Med 42:105-12
Yeo, Changmin; Son, Taeyoon; Park, Junghwan et al. (2010) Development of compression-controlled low-level laser probe system: towards clinical application. Lasers Med Sci 25:699-704
Huang, Yu-Chih; Tran, Nadia; Shumaker, Peter R et al. (2009) Blood flow dynamics after laser therapy of port wine stain birthmarks. Lasers Surg Med 41:563-71
Verkruysse, Wim; Svaasand, Lars O; Franco, Walfre et al. (2009) Remittance at a single wavelength of 390 nm to quantify epidermal melanin concentration. J Biomed Opt 14:014005
Izikson, Leonid; Nelson, J Stuart; Anderson, R Rox (2009) Treatment of hypertrophic and resistant port wine stains with a 755 nm laser: a case series of 20 patients. Lasers Surg Med 41:427-32
Son, Taeyoon; Han, Byungkwan; Jung, Byungjo et al. (2008) Fluorescent image analysis for evaluating the condition of facial sebaceous follicles. Skin Res Technol 14:201-7

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