Nanotechniques involve the creation, characterization, and modification of organized nanomaterials to serve as building blocks for the construction of large-scale devices and systems. Living systems contain a wide variety of nanomachines and highly ordered structures, including motors, arrays, pumps, membrane cores, and valves. The novelty and ingenious design of bacterial virus phi29 DNA packaging motor, the strongest biomotor studied to date, have inspired the synthesis of this motor and its components as biomemitics for nanodevices. This 30 nm nanomotor uses six ATP-binding pRNA molecules to gear the motor. An imitative motor has been successfully constructed using purified recombinant proteins and artificially synthesized RNA. It can be turned on and off at will. The formation of ordered structural arrays of the motor complex and its components, the retention of motor function after 3'-end extension of the pRNA, and the ease with which pRNA dimers and trimers can be manipulated combine to make the RNA-containing motor a viable option as mechanical parts in nanotechnology. The long-term objective is to utilize this synthesized motor, together with arrays of its components, as parts in nanodevices, with several major applications: 1) To use this artificial motor as parts for nanodevices, such as apparatuses for in vivo drug delivery. 2) To use the ordered arrays of motor components as building facades for large-scale supramolecular structures to serve as molecular sieves, chips for the diagnosis of diseases, or as ultrahigh density data storage systems. The presence of six pRNA subunits in the hexameric building blocks will allow for the construction of arrays with multiple functions, for example to allow for the separate identification of multiple pathogens. 3) To develop this nanomotor into a DNA-sequencing apparatus, since the DNA-packaging process involves movement of the DNA through a 3.6-nm pore surrounded by six RNA that can be modified to accept chemical signals. Though all of these long-term applications are becoming more and more realizable, practical nanotechnology is still in its infancy. It would be unrealistic to propose applying the phi29 motor system directly to nanodevices in only three or four years. Thus, the short-term objective of this proposal is to construct pRNA or protein arrays that will serve as templates for the construction of patterned supramolecular structures, and to find the best routes through which to link nanotubes and nanogolds as well as to attach biological moieties and chemical groups to the motor or the constructed arrays, pRNA dimers, trimers and hexamers will be used for array construction. Chief among early concerns will be avoiding disruption of the assembly and functioning of the motor and/or arrays due to the incorporation of foreign components. This phase of the study will pave the way toward direct and practical technological applications of the motor and its arrays in nanodevices.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
1R01EB003730-01
Application #
6794273
Study Section
Special Emphasis Panel (ZRG1-BPC-A (50))
Program Officer
Moy, Peter
Project Start
2004-09-01
Project End
2008-06-30
Budget Start
2004-09-01
Budget End
2005-06-30
Support Year
1
Fiscal Year
2004
Total Cost
$266,344
Indirect Cost
Name
Purdue University
Department
Type
Organized Research Units
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907
Pi, Fengmei; Zhang, Hui; Li, Hui et al. (2017) RNA nanoparticles harboring annexin A2 aptamer can target ovarian cancer for tumor-specific doxorubicin delivery. Nanomedicine 13:1183-1193
Binzel, Daniel W; Khisamutdinov, Emil; Vieweger, Mario et al. (2016) Mechanism of three-component collision to produce ultrastable pRNA three-way junction of Phi29 DNA-packaging motor by kinetic assessment. RNA 22:1710-1718
Binzel, Daniel W; Shu, Yi; Li, Hui et al. (2016) Specific Delivery of MiRNA for High Efficient Inhibition of Prostate Cancer by RNA Nanotechnology. Mol Ther 24:1267-77
Li, Hui; Lee, Taek; Dziubla, Thomas et al. (2015) RNA as a stable polymer to build controllable and defined nanostructures for material and biomedical applications. Nano Today 10:631-655
Shu, Dan; Pi, Fengmei; Wang, Chi et al. (2015) New approach to develop ultra-high inhibitory drug using the power function of the stoichiometry of the targeted nanomachine or biocomplex. Nanomedicine (Lond) 10:1881-97
Li, Hui; Rychahou, Piotr G; Cui, Zheng et al. (2015) RNA Nanoparticles Derived from Three-Way Junction of Phi29 Motor pRNA Are Resistant to I-125 and Cs-131 Radiation. Nucleic Acid Ther 25:188-97
Rychahou, Piotr; Shu, Yi; Haque, Farzin et al. (2015) Methods and assays for specific targeting and delivery of RNA nanoparticles to cancer metastases. Methods Mol Biol 1297:121-35
Zhang, Hui; Pi, Fengmei; Shu, Dan et al. (2015) Using RNA nanoparticles with thermostable motifs and fluorogenic modules for real-time detection of RNA folding and turnover in prokaryotic and eukaryotic cells. Methods Mol Biol 1297:95-111
Khisamutdinov, Emil F; Bui, My Nguyen Hoan; Jasinski, Daniel et al. (2015) Simple Method for Constructing RNA Triangle, Square, Pentagon by Tuning Interior RNA 3WJ Angle from 60° to 90° or 108°. Methods Mol Biol 1316:181-93
Haque, Farzin; Guo, Peixuan (2015) Overview of methods in RNA nanotechnology: synthesis, purification, and characterization of RNA nanoparticles. Methods Mol Biol 1297:1-19

Showing the most recent 10 out of 71 publications