Exposure of therapeutic and diagnostic medical devices to biological fluids is often accompanied by interfacial adsorption of proteins, cells and microorganisms. Biofouling of surfaces can lead to compromised device performance, increased cost, and in some cases may be life-threatening to the patient. The elimination or minimization of nonspecific biomolecule-material interactions is therefore an integral part of refining the biological performance of current and future biomaterials. Although several antifouling polymer coatings have enjoyed short-term success in preventing protein and cell adsorption on surfaces, none have proven ideal for conferring long-term biofouling resistance. The primary goal of this study is to design and synthesize novel long-lasting antifouling polymers with chemical and structural characteristics optimal for preventing protein fouling at biointerfaces. These polymers consist of two distinct domains coupled together- an anchoring domain inspired by the adhesive proteins secreted by mussels for attachment to marine surfaces, and an antifouling poly(N-substituted glycine) """"""""peptoid"""""""" segment designed to resist protein and cellular attachment. Peptidomimetic polymers with a variety of compositions, lengths, and architectures will be synthesized, and high sensitivity protein adsorption experiments will be performed to test the protein resistance of these polymers. The protein adsorption experiments will be both guided by, and confirmed with, theoretical calculations of the systems using a molecular theory that is particularly well-suited to investigating protein interactions with grafted polymers. Such systematic coupled experimental/theoretical investigations are difficult to accomplish with traditional synthetic polymers, but are facilitated in our case by the precise control of peptidomimetic polymer architecture, molecular weight, and composition. Outcomes of this study will include new insights into fundamental properties of antifouling polymers, as well as identification of new biologically inspired polymers capable of limiting protein and cell fouling of therapeutic and diagnostic device surfaces. PUBLIC HEALTH REVELANCE In this study we will combine theoretical and experimental approaches to study the antifouling properties of a new class of biomimetic polymers. When applied to the surface of an object, these polymers are anticipated to enhance the performance of medical devices by providing resistance to fouling by proteins, cells and bacteria.

National Institute of Health (NIH)
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
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Biomaterials and Biointerfaces Study Section (BMBI)
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Lee, Albert
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Northwestern University at Chicago
Biomedical Engineering
Schools of Engineering
United States
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Silies, Laura; Gonzalez Solveyra, Estefania; Szleifer, Igal et al. (2018) Insights into the Role of Counterions on Polyelectrolyte-Modified Nanopore Accessibility. Langmuir 34:5943-5953
Nap, Rikkert J; Gonzalez Solveyra, Estefania; Szleifer, Igal (2018) The interplay of nanointerface curvature and calcium binding in weak polyelectrolyte-coated nanoparticles. Biomater Sci 6:1048-1058
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Zhou, Jiajing; Xiong, Qirong; Ma, Jielin et al. (2016) Polydopamine-Enabled Approach toward Tailored Plasmonic Nanogapped Nanoparticles: From Nanogap Engineering to Multifunctionality. ACS Nano 10:11066-11075
Solveyra, Estefania Gonzalez; Tagliazucchi, Mario; Szleifer, Igal (2016) Anisotropic surface functionalization of Au nanorods driven by molecular architecture and curvature effects. Faraday Discuss 191:351-372
Gonzalez Solveyra, Estefania; Szleifer, Igal (2016) What is the role of curvature on the properties of nanomaterials for biomedical applications? Wiley Interdiscip Rev Nanomed Nanobiotechnol 8:334-54
Zhou, Jiajing; Wang, Peng; Wang, Chenxu et al. (2015) Versatile Core-Shell Nanoparticle@Metal-Organic Framework Nanohybrids: Exploiting Mussel-Inspired Polydopamine for Tailored Structural Integration. ACS Nano 9:6951-60
Zelasko-Leon, Daria C; Fuentes, Christina M; Messersmith, Phillip B (2015) MUC1-Targeted Cancer Cell Photothermal Ablation Using Bioinspired Gold Nanorods. PLoS One 10:e0128756

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