The primary aim of the proposed work is to develop microelectrodes for determining ifnasal membrane cholesterol is elevated in human cystic fibrosis. Studies will calibratethe electrodes through solution experiments and validate this calibration using a newproposed influx titration assay where the electrode is used as an indicator of influx.Human studies using the electrodes will involve single cell studies on epithelial cellsfrom nasal scrapes. In vivo electrode measurements in the human nose are alsoproposed. In developing the measurements as a diagnostic tool, implementation of invitro measurements at cells and mouse tissues is proposed to establish the relationshipbetween CFTR function and plasma membrane cholesterol content.

Public Health Relevance

Microelectrodes are used to measurer cholesterol efflux from cells andtissues. In vivo experiments are proposed in the human nose to test thehypothesis that cholesterol efflux rate can be used as a diagnostic tool in thestudy of human cystic fibrosis.

National Institute of Health (NIH)
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Research Project (R01)
Project #
Application #
Study Section
Enabling Bioanalytical and Imaging Technologies Study Section (EBIT)
Program Officer
Lash, Tiffani Bailey
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Georgia Regents University
United States
Zip Code
Lu, Binyu; Li, Li; Schneider, Molly et al. (2018) Electrochemical measurement of membrane cholesterol correlates with CFTR function and is HDAC6-dependent. J Cyst Fibros :
Li, Li; Lu, Binyu; Jiang, Dechen et al. (2017) Cell Plasma Membrane Cholesterol as a Diagnostic. Curr Opin Electrochem 2:82-87
Yu, Xiaochun; Kelley, Thomas J; Chiel, Hillel J et al. (2016) Communication-Microelectrode Detection of Cholesterol Efflux from the Human Buccel Mucosa. J Electrochem Soc 163:B453-B455
Rymut, Sharon M; Ivy, Tracy; Corey, Deborah A et al. (2015) Role of Exchange Protein Activated by cAMP 1 in Regulating Rates of Microtubule Formation in Cystic Fibrosis Epithelial Cells. Am J Respir Cell Mol Biol 53:853-62
West, Richard H; Lu, Hui; Shaw, Kendrick et al. (2014) Double Potential Pulse Chronocoulometry for Detection of Plasma Membrane Cholesterol Efflux at Disk Platinum Microelectrodes. J Electrochem Soc 161:B111-B116
Rymut, Sharon M; Harker, Alyssa; Corey, Deborah A et al. (2013) Reduced microtubule acetylation in cystic fibrosis epithelial cells. Am J Physiol Lung Cell Mol Physiol 305:L419-31
Manson, Mary E; Corey, Deborah A; Bederman, Ilya et al. (2012) Regulatory role of ?-arrestin-2 in cholesterol processing in cystic fibrosis epithelial cells. J Lipid Res 53:1268-76
Cianciola, Nicholas L; Carlin, Cathleen R; Kelley, Thomas J (2011) Molecular pathways for intracellular cholesterol accumulation: common pathogenic mechanisms in Niemann-Pick disease Type C and cystic fibrosis. Arch Biochem Biophys 515:54-63