The specific goals of this proposal are to develop state-of-the-art ultra-trace analytical methods for elemental analysis of environmentally important samples. Ultra-trace analyses will be developed with speciation, a necessity for reliable risk assessment. Chromatographic techniques will be combined with plasma source mass spectrometry (plasma MS) to provide elemental speciation for pollutant or potential pollutant inorganic, organic and organometallic compounds. By coupling plasma MS detection with supercritical fluid extraction and gas, liquid, or supercritical fluid chromatography (GC,LC, sometimes referred to as HPLC, or SFC, respectively), a variety of important environmental sample types can be determined at picomole and possibly lower levels. Detection after liquid chromatographic separations will be further enhanced through post-column hydride generation when appropriate. An additional attraction with plasma MS is that isotope information is simultaneously available. Studies will involve research involving ion formation and transport that will lead to better methods. While high resolution is expected from the chromatographic types employed, multiple element monitoring, with plasma MS selectivity, provides much additional information, since several chromatograms can be obtained from one sample injection (one for each elemental mass monitored). Additional plasma mass spectrometric methods will be initiated or further investigated. Since several plasma types have been investigated to date in these laboratories for their analytical utility, more fundamental characterization is necessary plus further studies to minimize matrix effects are warranted. Other studies will include glow discharge mass spectrometry for solid introduction of environmental samples and electrothermal vaporization for microliter volume sample introduction plus to minimize certain interferences. Also further investigation of plasmas for their potential to produce structural information as LC-MS sources to yield qualitative and quantitative results, will be undertaken. The importance of and approaches to these problems are detailed within.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Project (R01)
Project #
2R01ES003221-10
Application #
3250391
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1983-06-15
Project End
1995-05-31
Budget Start
1992-06-01
Budget End
1993-05-31
Support Year
10
Fiscal Year
1992
Total Cost
Indirect Cost
Name
University of Cincinnati
Department
Type
Schools of Arts and Sciences
DUNS #
City
Cincinnati
State
OH
Country
United States
Zip Code
45221
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Lin, L; Wang, J; Caruso, J (1995) Arsenic speciation using capillary zone electrophoresis with indirect ultraviolet detection. J Chromatogr Sci 33:177-80
Tomlinson, M J; Lin, L; Caruso, J A (1995) Plasma mass spectrometry as a detector for chemical speciation studies. Analyst 120:583-9
Kumar, U T; Vela, N P; Caruso, J A (1995) Multi-element detection of organometals by supercritical fluid chromatography with inductively coupled plasma mass spectrometric detection. J Chromatogr Sci 33:606-10
Carey, J M; Vela, N P; Caruso, J A (1994) Chromium determination by supercritical fluid chromatography with inductively coupled plasma mass spectrometric and flame ionization detection. J Chromatogr A 662:329-40
Kumar, U T; Dorsey, J G; Caruso, J A et al. (1993) Speciation of inorganic and organotin compounds in biological samples by liquid chromatography with inductively coupled plasma mass spectrometric detection. J Chromatogr A 654:261-8
Vela, N P; Olson, L K; Caruso, J A (1993) Elemental speciation with plasma mass spectrometry. Anal Chem 65:585A-597A
Pretty, J R; Blubaugh, E A; Caruso, J A (1993) Determination of arsenic(III) and selenium(IV) using an on-line anodic stripping voltammetry flow cell with detection by inductively coupled plasma atomic emission spectrometry and inductively coupled plasma mass spectrometry. Anal Chem 65:3396-403
Vela, N P; Caruso, J A (1993) Comparison of flame ionization and inductively coupled plasma mass spectrometry for the detection of organometallics separated by capillary supercritical fluid chromatography. J Chromatogr 641:337-45
Sheppard, B S; Caruso, J A; Heitkemper, D T et al. (1992) Arsenic speciation by ion chromatography with inductively coupled plasma mass spectrometric detection. Analyst 117:971-5

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