Abstract

A proposal is made to develop quantitative 3-dimensional magnetic resonance imaging (MRI) with microscopic resolution as an in vivo assay for the response of small animals to environmental toxins. Specifically the proposal will develop MR pulse sequences for accurate determination of spin-lattice (T1) and spin-spin (T2) relaxation times over 8-32 contiguous 1 mm thick slices with resolution of less than 100 microns. The improved accuracy and high resolution will remove the systematic errors and volume averaging that have limited the specificity of MRI in previous studies. The sequences optimized for small animal imaging will be applied to studies of three environmental toxins in the rat: carbon tetrachloride, alpha-naphthlisothiocyanate, and diethyl nitrosamine. Histogram analysis of T1/T2 images will be correlated with morphometric histologic examination to determine the sensitivity and specificity of MR in evaluating fatty change, fibrosis, necrosis, and neoplasia in the liver. The methods developed will replace the more costly and time consuming techniques of necropsy. Of particular interest is the fact that the techniques do not require sacrifice of the animal. Thus, the time course of a study can be followed in a single animal, reducing the number of animals required and permitting the study of progression and regression in the toxic response. The work promises a new approach to the study of toxicology using in vivo histology.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Project (R01)
Project #
5R01ES004187-03
Application #
3252171
Study Section
Diagnostic Radiology Study Section (RNM)
Project Start
1987-04-15
Project End
1991-03-31
Budget Start
1989-04-01
Budget End
1991-03-31
Support Year
3
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
Duke University
Department
Type
Schools of Medicine
DUNS #
071723621
City
Durham
State
NC
Country
United States
Zip Code
27705

  Publications

Zhou, X; Liang, Z P; Gewalt, S L et al. (1998) A fast spin echo technique with circular sampling. Magn Reson Med 39:23-7
Zhou, X; Maronpot, R R; Hedlund, L W et al. (1995) Detection of bromobenzene-induced hepatocellular necrosis using magnetic resonance microscopy. Magn Reson Med 34:853-7
Zhou, X; Maronpot, R R; Cofer, G P et al. (1994) Studies on bromobenzene-induced hepatotoxicity using in vivo MR microscopy with surgically implanted RF coils. Magn Reson Med 31:619-27
Black, R D; Early, T A; Roemer, P B et al. (1993) A high-temperature superconducting receiver for nuclear magnetic resonance microscopy. Science 259:793-5
Gewalt, S L; Glover, G H; Hedlund, L W et al. (1993) MR microscopy of the rat lung using projection reconstruction. Magn Reson Med 29:99-106
Beaulieu, C F; Zhou, X; Cofer, G P et al. (1993) Diffusion-weighted MR microscopy with fast spin-echo. Magn Reson Med 30:201-6
Zhou, X; Liang, Z P; Cofer, G P et al. (1993) Reduction of ringing and blurring artifacts in fast spin-echo imaging. J Magn Reson Imaging 3:803-7
Zhou, X; Cofer, G P; Suddarth, S A et al. (1993) High-field MR microscopy using fast spin-echoes. Magn Reson Med 30:60-7
Banson, M L; Cofer, G P; Black, R et al. (1992) A probe for specimen magnetic resonance microscopy. Invest Radiol 27:157-64
Brouwer, M; Engel, D W; Bonaventura, J et al. (1992) In vivo magnetic resonance imaging of the blue crab, Callinectes sapidus: effect of cadmium accumulation in tissues on proton relaxation properties. J Exp Zool 263:32-40

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