Through their ability to alter gene expression and affect cell proliferation and differentiation, 2,3,7,8-tetra-chlorodibenzo-p-dioxin (TCDD) and related xenobiotics are potent immunogenic, reproductive, and developmental toxicants. They produce these effects by binding to a gene regulatory protein, the Ah receptor (AhR), to inappropriately modulate gene expression. It is not known which developing tissues are most sensitive to these compounds and how this sensitivity varies during critical developmental periods and/or during critical times of exposure. The normal function of the AhR has not been delineated and an endogenous ligand has not been identified. Through a determination of the tissue- specific transcriptional activity of the AhR complex in vivo it will be possible to determine: 1) in which developing issues the AhR complex has normal function, and 2) in which tissues the exposure to TCDD may disrupt this process. Thus, it will be possible to determine the target tissues for TCDD toxicity and the critical periods of development that are most sensitive. The purpose of this project is to determine the tissue and cellular sites and time of AhR stimulated transcription activity in developing animals utilizing a transgenic mouse model. A developed hemizygous mouse model will be bred to homozygosity. The presence and inheritability of the transgene will be determined by RT-PCR and Southern blotting. Utilizing various mouse models containing a responsive transgene, studies will determine whether the developmental expression of the transgene is or is not dependent on the site of gene integration. Studies will examine the ability of the transgene to be induced following exposure of young adult animals to TCDD. These data will be compared to the level of transcriptionally active AhR complete and the inducibility of other genes. Dose-response studies will determine sensitivity. These studies will verify that the expression of the transgene is related to the presence of the active AhR. By sensitive in situ staining techniques, the developmental expression of the transgene in tissues and cells will be determined in the absence of TCDD exposure. Results will be compared to immunohistochemical studies to determine the presence of AhR protein and its heterodimeric partner, Arnt, in these tissues. Additional studies will determine the modulated expression of the transgene following exposure of developing animals to TCDD.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Project (R01)
Project #
5R01ES009430-02
Application #
6150735
Study Section
Chemical Pathology Study Section (CPA)
Program Officer
Heindel, Jerrold
Project Start
1999-02-01
Project End
2002-01-31
Budget Start
2000-02-01
Budget End
2001-01-31
Support Year
2
Fiscal Year
2000
Total Cost
$200,876
Indirect Cost
Name
University of Rochester
Department
Public Health & Prev Medicine
Type
Schools of Dentistry
DUNS #
208469486
City
Rochester
State
NY
Country
United States
Zip Code
14627
Vezina, Chad M; Allgeier, Sarah Hicks; Moore, Robert W et al. (2008) Dioxin causes ventral prostate agenesis by disrupting dorsoventral patterning in developing mouse prostate. Toxicol Sci 106:488-96
Gasiewicz, Thomas A; Henry, Ellen C; Collins, Loretta L (2008) Expression and activity of aryl hydrocarbon receptors in development and cancer. Crit Rev Eukaryot Gene Expr 18:279-321
Collins, Loretta L; Williamson, Mary A; Thompson, Bryan D et al. (2008) 2,3,7,8-Tetracholorodibenzo-p-dioxin exposure disrupts granule neuron precursor maturation in the developing mouse cerebellum. Toxicol Sci 103:125-36
Bemis, Jeffrey C; Alejandro, Napoleon F; Nazarenko, Daniel A et al. (2007) TCDD-induced alterations in gene expression profiles of the developing mouse paw do not influence morphological differentiation of this potential target tissue. Toxicol Sci 95:240-8
Ryan, Elizabeth P; Holz, Jonathan D; Mulcahey, Mary et al. (2007) Environmental toxicants may modulate osteoblast differentiation by a mechanism involving the aryl hydrocarbon receptor. J Bone Miner Res 22:1571-80
Thatcher, Thomas H; Maggirwar, Sanjay B; Baglole, Carolyn J et al. (2007) Aryl hydrocarbon receptor-deficient mice develop heightened inflammatory responses to cigarette smoke and endotoxin associated with rapid loss of the nuclear factor-kappaB component RelB. Am J Pathol 170:855-64
Henry, E C; Bemis, J C; Henry, O et al. (2006) A potential endogenous ligand for the aryl hydrocarbon receptor has potent agonist activity in vitro and in vivo. Arch Biochem Biophys 450:67-77
Williamson, Mary A; Gasiewicz, Thomas A; Opanashuk, Lisa A (2005) Aryl hydrocarbon receptor expression and activity in cerebellar granule neuroblasts: implications for development and dioxin neurotoxicity. Toxicol Sci 83:340-8
Bemis, Jeffrey C; Nazarenko, Daniel A; Gasiewicz, Thomas A (2005) Coplanar polychlorinated biphenyls activate the aryl hydrocarbon receptor in developing tissues of two TCDD-responsive lacZ mouse lines. Toxicol Sci 87:529-36
Filbrandt, Carissa R; Wu, Zhenhua; Zlokovic, Berislav et al. (2004) Presence and functional activity of the aryl hydrocarbon receptor in isolated murine cerebral vascular endothelial cells and astrocytes. Neurotoxicology 25:605-16

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