Environmental exposures to toxic or carcinogenic compounds rarely result from the action of single toxicants. More often, the toxic agent is a complex mixture of chemical entities in numbers ranging from a few, such as in many occupational exposures, to several thousand, as in cigarette smoke. Frequently, these mixtures include a combination of carcinogenic metals and polycyclic aromatic hydrocarbons (PAH). Of these, hexavalent chromium, a carcinogenic metal often found as an environmental co-contaminant with PAHs, and benzo[a]pyrene (BaP), a prototypical PAH, are among the top 20 hazardous substances in the ATSDR/EPA priority list. The long-range goal of the research funded by this grant has been to develop an understanding of the mechanisms responsible for the adverse health effects of exposure to binary mixtures of BaP and chromium(VI). We have shown that high-dose acute chromium treatment interferes with the assembly of productive transcriptional complexes by cross-linking HDAC1.DNMT1 complexes to promoter chromatin and inhibiting phosphorylation, acetylation and methylation epigenetic marks established by BaP-induced gene transactivation in histones H3 and H4. These changes inhibit recruitment of RNA polymerase II to the Cyp1a1 promoter, block BaP-inducible gene expression and stimulate the formation of BPDE-DNA adducts. Similarly, sustained exposure to low doses of chromium leads to gradual changes in histone marks and signal transduction pathways that cumulatively affect gene expression and silencing. Specifically, environmentally relevant doses of chromium(VI) have opposite effects on the BaP-mediated induction of CYP1A1, CYP1B1 and CYP1A2 cytochrome P450 genes, repress tumor suppressor gene expression and induce expression of pro-apoptotic genes, leading us to the hypothesis that environmentally relevant binary mixtures of chromium(VI) and BaP have a synergistic effect on BaP toxicity and carcinogenicity. Based on our findings, the objectives for the next 5 years of this grant are, (1) to elucidate the epigenetic mechanisms by which low-dose, long-term exposure to chromium affects BaP-inducible gene expression;(2) to evaluate how chronic exposure to environmentally relevant chromium doses in drinking water affects BaP toxicity and carcinogenicity. Epigenetic modification of gene expression is a key element of the developmental and carcinogenic outcomes of exposure to chromium, alone or in combination with PAHs. Hypermethylation of the promoter of the tumor suppressor p16ink4a gene has been found in chromium-exposed workers who developed lung cancer, although p16ink4a promoter methylation accounts for only one third of cancer in this population, suggesting that there may be additional chromium targets and alternative mechanisms underlying cancer development. The knowledge derived from this research will have a major impact on the medical translation of these epidemiological findings and, by identifying molecular targets useful to reduce disease incidence, will significantly contribute to the development of therapeutic and preventative measures.
Environmental exposure to hexavalent chromium in drinking water and cigarette smoking and occupational exposure in the workplace are often compounded with concomitant exposures to aromatic hydrocarbon procarcinogens, resulting in health problems including lung, stomach and intestinal tract tumors. The objective of this grant proposal is to evaluate how mixtures of hexavalent chromium and aromatic hydrocarbons cooperate to cause greater toxicity and carcinogenicity than the sum of the toxicities and carcinogenicities of the two compounds. The knowledge derived from this research will identify molecular targets to reduce disease incidence and will significantly contribute to the development of therapeutic and preventative measures with major impact on the treatment of the diseases caused by these agents.
|VonHandorf, Andrew; Sánchez-Martín, Francisco Javier; Biesiada, Jacek et al. (2018) Chromium disrupts chromatin organization and CTCF access to its cognate sites in promoters of differentially expressed genes. Epigenetics 13:363-375|
|Ko, Chia-I; Puga, Alvaro (2017) Does the Aryl Hydrocarbon Receptor Regulate Pluripotency? Curr Opin Toxicol 2:1-7|
|Wang, Qin; Kurita, Hisaka; Carreira, Vinicius et al. (2016) Ah Receptor Activation by Dioxin Disrupts Activin, BMP, and WNT Signals During the Early Differentiation of Mouse Embryonic Stem Cells and Inhibits Cardiomyocyte Functions. Toxicol Sci 149:346-57|
|Ko, Chia-I; Fan, Yunxia; de Gannes, Matthew et al. (2016) Repression of the Aryl Hydrocarbon Receptor Is Required to Maintain Mitotic Progression and Prevent Loss of Pluripotency of Embryonic Stem Cells. Stem Cells 34:2825-2839|
|Carreira, Vinicius S; Fan, Yunxia; Wang, Qing et al. (2015) Ah Receptor Signaling Controls the Expression of Cardiac Development and Homeostasis Genes. Toxicol Sci 147:425-35|
|Carreira, Vinicius S; Fan, Yunxia; Kurita, Hisaka et al. (2015) Disruption of Ah Receptor Signaling during Mouse Development Leads to Abnormal Cardiac Structure and Function in the Adult. PLoS One 10:e0142440|
|Sánchez-Martín, Francisco Javier; Lindquist, Diana M; Landero-Figueroa, Julio et al. (2015) Sex- and tissue-specific methylome changes in brains of mice perinatally exposed to lead. Neurotoxicology 46:92-100|
|Sánchez-Martín, Francisco Javier; Fan, Yunxia; Carreira, Vinicius et al. (2015) Long-term Coexposure to Hexavalent Chromium and B[a]P Causes Tissue-Specific Differential Biological Effects in Liver and Gastrointestinal Tract of Mice. Toxicol Sci 146:52-64|
|Ovesen, Jerald L; Fan, Yunxia; Chen, Jing et al. (2014) Long-term exposure to low-concentrations of Cr(VI) induce DNA damage and disrupt the transcriptional response to benzo[a]pyrene. Toxicology 316:14-24|
|Ko, Chia-I; Wang, Qin; Fan, Yunxia et al. (2014) Pluripotency factors and Polycomb Group proteins repress aryl hydrocarbon receptor expression in murine embryonic stem cells. Stem Cell Res 12:296-308|
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