Background: Increasing epidemiologic and experimental evidence supports the role of inorganic arsenic in the development of diabetes. Arsenic in drinking water is a major concern for US populations who live in small rural communities. Prospective evidence and mechanisms for arsenic related diabetes must be investigated. Objective: To evaluate the role of inorganic arsenic exposure and metabolism and gene-arsenic interactions in the development of diabetes, insulin resistance, ? cell dysfunction and the metabolic syndrome among 2,717 Strong Heart Family Study (SHFS) participants e18 year old who were free of diabetes at baseline. Preliminary studies: We have identified an association between urine arsenic concentrations and the prevalence of diabetes in American Indians 45-74 year old from Arizona, Oklahoma and the Dakotas who participated in the Strong Heart Study (SHS). Urine arsenic concentrations and methylation excretion patterns remained constant over a 10-year follow-up. The heritability of the proportion of monomethylarsonate (%MMA) in urine was 0.52. In a small linkage study, we detected potential quantitative trait loci associated with %MMA in areas of the genome close to methyltransferase genes. Design and setting: The SHFS is a population- based prospective family study that recruited parents, spouses, offspring, spouses of offspring and grandchildren of SHS participants in 1998-2005. Demographic, lifestyle and medical information and measures of fasting glucose and insulin, lipid profile, hypertension endpoints, body mass index, and waist and hip circumferences are available at baseline and follow-up visits through 2011. Urine samples and blood DNA samples were stored at -70oC. Exposure assessment: We will measure total urine arsenic and urine arsenic species concentrations using inductively coupled plasma-mass spectrometry (ICPMS) and high performance liquid chromatography-ICPMS (HPLC-ICPMS), respectively. Genetic assessment: We will prioritize and measure ~1,500 SNPs in candidate genes related to arsenic metabolism and toxicity using a multiplex panel. We will measure SNPs in candidate genes related to diabetes traits using the Metabo-chip, a highly cost- effective candidate gene chip for diabetes and cardiometabolic traits. Statistical analysis: We will use mixed- effect models for binary and linear outcomes to assess the prospective association of urine arsenic species concentrations at baseline with the incidence of diabetes and the metabolic syndrome and changes in the homeostatic model assessment to quantify insulin resistance (HOMA-IR) and ? cell dysfunction (HOMA-B) over time. Gene-arsenic interaction will be estimated using principle component analysis and accounting for population stratification using genomic control. Significance: By investigating the contribution of inorganic arsenic to diabetes, insulin resistance, ? cell dysfunction and the metabolic syndrome and by identifying genetic susceptibility factors, this study can inform the arsenic-diabetes relationship and impact the prevention and control of arsenic exposure in drinking water and food in the US and abroad.

Public Health Relevance

Increasing evidence supports the role of arsenic exposure in the development of diabetes. This study will evaluate the role of arsenic exposure and metabolism and their interaction with genetic markers in the development of diabetes in American Indian communities. This study can provide insight into the arsenic- diabetes relationship and inform public health recommendations for arsenic levels in drinking water and food.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Project (R01)
Project #
1R01ES021367-01
Application #
8266205
Study Section
Special Emphasis Panel (ZRG1-EMNR-D (55))
Program Officer
Heindel, Jerrold
Project Start
2012-07-01
Project End
2016-03-31
Budget Start
2012-07-01
Budget End
2013-03-31
Support Year
1
Fiscal Year
2012
Total Cost
$688,401
Indirect Cost
$157,262
Name
Johns Hopkins University
Department
Public Health & Prev Medicine
Type
Schools of Public Health
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Oliver-Williams, Clare; Howard, Annie Green; Navas-Acien, Ana et al. (2018) Cadmium body burden, hypertension, and changes in blood pressure over time: results from a prospective cohort study in American Indians. J Am Soc Hypertens 12:426-437.e9
Jones, Miranda R; Tellez-Plaza, Maria; Vaidya, Dhananjay et al. (2018) Ethnic, geographic and dietary differences in arsenic exposure in the multi-ethnic study of atherosclerosis (MESA). J Expo Sci Environ Epidemiol :
Balakrishnan, Poojitha; Vaidya, Dhananjay; Voruganti, V Saroja et al. (2018) Genetic Variants Related to Cardiometabolic Traits Are Associated to B Cell Function, Insulin Resistance, and Diabetes Among AmeriCan Indians: The Strong Heart Family Study. Front Genet 9:466
Sanchez, Tiffany R; Powers, Martha; Perzanowski, Matthew et al. (2018) A Meta-analysis of Arsenic Exposure and Lung Function: Is There Evidence of Restrictive or Obstructive Lung Disease? Curr Environ Health Rep 5:244-254
Spratlen, Miranda J; Grau-Perez, Maria; Umans, Jason G et al. (2018) Arsenic, one carbon metabolism and diabetes-related outcomes in the Strong Heart Family Study. Environ Int 121:728-740
Balakrishnan, Poojitha; Navas-Acien, Ana; Haack, Karin et al. (2018) Arsenic-gene interactions and beta-cell function in the Strong Heart Family Study. Toxicol Appl Pharmacol 348:123-129
Nigra, Anne E; Nachman, Keeve E; Love, David C et al. (2017) Poultry Consumption and Arsenic Exposure in the U.S. Population. Environ Health Perspect 125:370-377
Moon, Katherine A; Oberoi, Shilpi; Barchowsky, Aaron et al. (2017) A dose-response meta-analysis of chronic arsenic exposure and incident cardiovascular disease. Int J Epidemiol 46:1924-1939
Nachman, Keeve E; Love, David C; Baron, Patrick A et al. (2017) Nitarsone, Inorganic Arsenic, and Other Arsenic Species in Turkey Meat: Exposure and Risk Assessment Based on a 2014 U.S. Market Basket Sample. Environ Health Perspect 125:363-369
Grau-Perez, Maria; Kuo, Chin-Chi; Gribble, Matthew O et al. (2017) Association of Low-Moderate Arsenic Exposure and Arsenic Metabolism with Incident Diabetes and Insulin Resistance in the Strong Heart Family Study. Environ Health Perspect 125:127004

Showing the most recent 10 out of 45 publications