Abstract

The applicant proposes to examine excitatory and inhibitory receptors in bipolar cells, using whole cell and perforated-patch electrophysiological methods in both the isolated cell and slice preparations of the skate retina. One goal will be to provide a detailed description of the effects of zinc on GABA-A and GABA-C receptors and determine how this influences synaptic transmission between photoreceptors and bipolar cells. The second goal will be to extend studies that will establish that there are ON and OFF bipolars in the skate retina. The types of glutamate receptors on bipolar cells will be examined and compared to immunohistochemical, morphological markers.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
2R01EY000777-21A1
Application #
2019376
Study Section
Visual Sciences C Study Section (VISC)
Project Start
1985-09-30
Project End
2000-08-31
Budget Start
1997-09-01
Budget End
1998-08-31
Support Year
21
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Hunter College
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10065

  Publications

Rosenstein, Frederick J; Chappell, Richard L (2003) Endogenous zinc as a retinal neuromodulator: evidence from the skate (Raja erinacea). Neurosci Lett 345:81-4
Redenti, Stephen; Chappell, Richard L (2002) Zinc chelation enhances the zebrafish retinal ERG b-wave. Biol Bull 203:200-2
Chappell, Richard L; Schuette, Etha; Anton, Robert et al. (2002) GABA(C) receptors modulate the rod-driven ERG b-wave of the skate retina. Doc Ophthalmol 105:179-88
Chappell, R L (2001) Retinal information processing and ambient illumination. Prog Brain Res 131:177-84
Chappell, R L; Redenti, S (2001) Endogenous zinc as a neuromodulator in vertebrate retina: evidence from the retinal slice. Biol Bull 201:265-7
Qian, H; Ripps, H; Schuette, E et al. (2001) Responses of small- and large-field bipolar cells to GABA and glycine. Brain Res 893:273-7
Schuette, E; Chappell, R L (1998) Excitatory amino acids and serotonin uptake blockers reveal two physiologically distinct serotonin systems in the retina of the skate, Raja erinacea. Int J Neurosci 95:115-32
Qian, H; Li, L; Chappell, R L et al. (1997) GABA receptors of bipolar cells from the skate retina: actions of zinc on GABA-mediated membrane currents. J Neurophysiol 78:2402-12
Qian, H; Malchow, R P; Chappell, R L et al. (1996) Zinc enhances ionic currents induced in skate Muller (glial) cells by the inhibitory neurotransmitter GABA. Proc Biol Sci 263:791-6
Schlemermeyer, E; Chappell, R L (1996) Two classes of bipolar cell in the retina of the skate Raja erinacea. J Neurocytol 25:625-35

Showing the most recent 10 out of 17 publications