Experiments are presented which examine the interaction between the retina and the pigment epithelium. In particular, we will investigate the molecular biology of the balanced process of outer segment renewal and shedding which is responsible for maintaining the outer segments at a constant length. The overall goal of this application is to better understand the regulation of renewal, shedding, and phagocytosis in frogs maintained on a controlled diurnal lighting cycle, and to describe the effect of alterations in the normal cycle. Using the frog as a model and the tehcniques of microscopy, biochemistry, and autoradiography, we will examine in detail outer segment renewal, shedding and phagocytosis. Specifically, both in vivo and in vitro experiments, renewal and shedding will be studied in order to determine: 1) what primes photoreceptors to shed, 2) how does light induce shedding, 3) what is the primary site of light reception, 4) does the neuroendocrine system play a role in renewal and shedding, 5) during shedding, what changes occur which make the shed tip recoginzed and phagocytized by the pigment epithelium, 6) what morphological and biochemical changes occur within the pigment epithelium during shedding and phagocytosis of outer segment tips, and 7) what is the role of phospholipids in these processes? These experiments should provide important information on the normal function of the retina and pigment epithelium, and may give us further insight into how these two tissues operate together to perform the complex metabolic and physiological functions which characterize a normal, healthy retina.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY001406-12
Application #
3255928
Study Section
(VID)
Project Start
1977-07-01
Project End
1988-06-30
Budget Start
1986-07-01
Budget End
1988-06-30
Support Year
12
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Baylor College of Medicine
Department
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030