Abstract

Freshly isolated rabbit lenses were incubated in different culture mediums. It was found that the alteration of molarity of the medium (hypotonic or hypertonic) significantly inhibits protein metabolism; similar results were also obtained when the pH of the medium was varied. Hypertonic culture medium can induce temporary superficial cortical fiber opacity. Alpha-crystallin has a half-life of more than three days.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY001848-09
Application #
3256248
Study Section
(SSS)
Project Start
1976-09-30
Project End
1988-08-31
Budget Start
1986-09-01
Budget End
1988-08-31
Support Year
9
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Wayne State University
Department
Type
Schools of Medicine
DUNS #
City
Detroit
State
MI
Country
United States
Zip Code
48202

  Publications

Niyogi, T K; Emanuel, K; Parafina, J et al. (1991) The effects of iris-ciliary complex on the organ cultured rabbit ocular lens. Lens Eye Toxic Res 8:43-60
Banerjee, A; Richiert, D M; Emanuel, K et al. (1991) Studies on the possible role of vitreous humor on protein synthesis and morphology of organ cultured adult rabbit lens. II. Epithelial cells. Biochim Biophys Acta 1076:330-6
Banerjee, A; Richiert, D M; Bagchi, M (1991) Phosphorylation of small molecular weight polypeptides in the iris-ciliary complex, aqueous humor and vitreous humor. Biochim Biophys Acta 1077:56-64
Singh, A K; Bagchi, M (1989) Studies on the possible role of vitreous humor on the protein synthesis and clarity of the organ cultured ocular lens. Lens Eye Toxic Res 6:823-31
Bagchi, M; Caporale, M J; Dave, A A et al. (1988) The role of the iris-ciliary complex in the control of protein synthesis by the organ cultured rabbit ocular lens. Exp Eye Res 46:897-907
Kiessling, B; Barret, R; Caporale, M J et al. (1988) Study of experimental cataract produced by sugar alcohols in the organ-cultured mammalian ocular lens. Ophthalmic Res 20:237-44
Bagchi, M; Dave, A A; Singh, A K et al. (1987) Iris-ciliary complex inhibits protein synthesis by organ cultured mammalian ocular lens cells. Invest Ophthalmol Vis Sci 28:759-62
Bagchi, M; Caporale, M J; Wechter, R S et al. (1985) Vimentin synthesis by ocular lens cells. Exp Eye Res 40:385-92
Bagchi, M; Caporale, M J; Gordon, S R (1985) Effect of hyperosmolar medium on the protein synthesis and morphology of epithelial cells of the cultured rabbit lens. Exp Eye Res 40:499-508