Abstract

The proposed program for next grant period is concerned with: 1. Steady State levels of cations, kinetic parameters of Rb86 uptake and efflux from the lens as affected by X-irradiation; the changes in ion transport will be correlated with lens ATP levels and changes in the activities of membrane ATPase. 2. Immunoelectrophoretic studies on the loss of soluble proteins from X-rayed lens into aqueous humor. 3. The effect of X-ray on the levels of ascorbic acid and H2O2 in aqueous humor and lens of rabbit. 4. The isolation and further characterization of crystallins which are released from X-ray induced protein aggregates by treatment with sulfhydryl reducing agents.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY002027-09
Application #
3256404
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1977-08-01
Project End
1986-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
9
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Oakland University
Department
Type
Organized Research Units
DUNS #
City
Rochester
State
MI
Country
United States
Zip Code
48309

  Publications

Cencer, Caroline S; Chintala, Shravan K; Townsend, Tenira J et al. (2018) PARP-1/PAR Activity in Cultured Human Lens Epithelial Cells Exposed to Two Levels of UVB Light. Photochem Photobiol 94:126-138
Raju, Murugesan; Mooney, Brian P; Thakkar, Kavi M et al. (2015) Role of ?A-crystallin-derived ?A66-80 peptide in guinea pig lens crystallin aggregation and insolubilization. Exp Eye Res 132:151-60
Padgaonkar, Vanita A; Leverenz, Victor R; Bhat, Aparna V et al. (2015) Thioredoxin reductase activity may be more important than GSH level in protecting human lens epithelial cells against UVA light. Photochem Photobiol 91:387-96
Lyons, Brian; Karuso, Peter; Jamie, Joanne F et al. (2014) Characterisation of a novel UV filter in the lens of the thirteen-lined ground squirrel (Ictidomys tridecemlineatus). Exp Eye Res 121:114-20
Giblin, Frank J; David, Larry L; Wilmarth, Phillip A et al. (2013) Shotgun proteomic analysis of S-thiolation sites of guinea pig lens nuclear crystallins following oxidative stress in vivo. Mol Vis 19:267-80
Giblin, Frank J; Lin, Li-Ren; Simpanya, Mukoma F et al. (2012) A Class I UV-blocking (senofilcon A) soft contact lens prevents UVA-induced yellow fluorescence and NADH loss in the rabbit lens nucleus in vivo. Exp Eye Res 102:17-27
Giblin, Frank J; Lin, Li-Ren; Leverenz, Victor R et al. (2011) A class I (Senofilcon A) soft contact lens prevents UVB-induced ocular effects, including cataract, in the rabbit in vivo. Invest Ophthalmol Vis Sci 52:3667-75
Goldenberg, David T; Giblin, Frank J; Cheng, Mei et al. (2011) Posterior vitreous detachment with microplasmin alters the retinal penetration of intravitreal bevacizumab (Avastin) in rabbit eyes. Retina 31:393-400
Simpanya, Mukoma F; Leverenz, Victor R; Giblin, Frank J (2010) Expression and purification of his-tagged recombinant mouse zeta-crystallin. Protein Expr Purif 69:147-52
Giblin, F J; Quiram, P A; Leverenz, V R et al. (2009) Enzyme-induced posterior vitreous detachment in the rat produces increased lens nuclear pO2 levels. Exp Eye Res 88:286-92

Showing the most recent 10 out of 67 publications