This project is directed at characterizing the time of appearance of specific neurotransmitter properties during development of the vertebrate retina. It will utilize biochemical, autoradiographic, morphological and physiological techniques to follow the time of appearance of the high affinity uptake for specific neurotransmitters, the time when neurotransmitters are first synthesized during development, time when release of neurotransmitters can first be evoked and the time of post-synaptic receptor appearance. Using dissociated neuroepithelial cells derived from retinal rudiments from the embryo maintained in tissue culture, we will determine the requirements for cell-to-cell contacts during retinal differentiation for the appearance of specific neurotransmitter properties. The project will also involve an analysis of the factors which regulate membrane assembly in photoreceptors. It will systematically evaluate the involvement of cyclic nucleotide metabolism, phosphorylation reactions, and calcium fluxes in establishing the burst of membrane assembly at the base of rod outer segments which follows the onset of the light cycle. The project will also evaulate the involvement of environmental lighting in setting the rates of metabolism of RNA and protein synthesis within the retina. A basic understanding of the regulation of retinal development, renewal processes and rates of retinal metabolism will provide important new information to understanding the control of these processes in retinal health and disease.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY002363-10
Application #
3256726
Study Section
(VID)
Project Start
1977-07-01
Project End
1987-08-31
Budget Start
1986-03-01
Budget End
1987-08-31
Support Year
10
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Baylor College of Medicine
Department
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030
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Al-Ubaidi, M R; Mangini, N J; Quiambao, A B et al. (1997) Unscheduled DNA replication precedes apoptosis of photoreceptors expressing SV40 T antigen. Exp Eye Res 64:573-85
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Frederick, J M; Rayborn, M E; Hollyfield, J G (1989) Serotoninergic neurons in the retina of Xenopus laevis: selective staining, identification, development, and content. J Comp Neurol 281:516-31

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