Abstract

The aims of this project are to develop animal models of immediate and delayed conjunctival hypersensitivity and to define their clinical and histologic appearance. Immunologic studies will be carried out to determine the humoral and cellular immune components of these reactions. Drugs which may inhibit the inflammatory reactions will be used to prevent and treat these models of conjunctival hypersensitivity. Immediate hypersensitivity will be induced in the guinea pig by intradermal or intravitreal injection, or by topical conjunctival application of ovalbumin. Animals will be challenged by conjunctival application of ovalbumin two weeks after sensitization. Delayed conjunctival hypersensitivity will be induced with the contact sensitizer oxazalone and conjunctival challenge will be performed one to two weeks after sensitization. Conjunctival reactions will be evaluated clinically and with fluorophotometry or radioisotope scanning. The cellular components of the inflammatory reactions will be evaluated histologically using JB-4 embedded 1 micron sections. Langerhans' cell activity in the corneal epithelium will be evaluated with the ATPase stain. Anti-inflammatory agents will be administered before and after conjunctival challenge, and in some instances before sensitization. The long-term objectives of these experiments is to develop an animal model for human ocular allergy, to define the immunopathology of human ocular allergic disease, and to determine if specific drugs or drug classes are efficacious in the treatment of ocular allergy.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY002502-11
Application #
3256819
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1987-01-01
Project End
1989-06-30
Budget Start
1987-07-01
Budget End
1989-06-30
Support Year
11
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Scripps Research Institute
Department
Type
DUNS #
City
San Diego
State
CA
Country
United States
Zip Code
92037

  Publications

Chen, Hung-Chi; Zhu, Ying-Ting; Chen, Szu-Yu et al. (2012) Wnt signaling induces epithelial-mesenchymal transition with proliferation in ARPE-19 cells upon loss of contact inhibition. Lab Invest 92:676-87
Proud, D; Sweet, J; Stein, P et al. (1990) Inflammatory mediator release on conjunctival provocation of allergic subjects with allergen. J Allergy Clin Immunol 85:896-905
Hodges, E J; Friedlaender, M H; Lee, A et al. (1989) Effect of minimal antibiotic treatment on bacterial keratitis. Cornea 8:188-90
Friedlaender, M H (1989) Conjunctival provocative tests: a model of human ocular allergy. Trans Am Ophthalmol Soc 87:577-97
Friedlaender, M H; Lee, A; Manka, R L (1988) Laboratory diagnosis of the allergic eye. Clin Rev Allergy 6:341-56
Friedlaender, M H (1988) Corneal biopsy. Int Ophthalmol Clin 28:101-2
Friedlaender, M H; Cameron, J (1988) Vernal keratoconjunctivitis and trachoma. Int Ophthalmol 12:47-51
Alvarez, R A; Liou, G I; Fong, S L et al. (1987) Levels of alpha- and gamma-tocopherol in human eyes: evaluation of the possible role of IRBP in intraocular alpha-tocopherol transport. Am J Clin Nutr 46:481-7
Fong, S L; Liou, G I; Bridges, C D (1986) Purification of interstitial retinol-binding protein from the eye. Methods Enzymol 123:102-11
Friedlaender, M H (1986) Ocular allergy. Scratching the surface of the red eye. Postgrad Med 79:261-71

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