Numerous clinical and experimental observations have suggested that the eye is an immunologically unique organ. We have endeavored to study the immunologic characteristics of the eye to better understand the immune pathogenesis of intraocular inflammatory disorders. In this regard we have studied the systemic immune response following the presentation of antigen via the anterior chamber (AC), a phenomenon termed anterior chamber-associated immune deviation (ACAID). The seminal observation in ACAID is that the systemic immune response is modulated by intraocular events following the entrance of antigen into the eye; e.g., as would occur in intraocular infectious diseases. Our recent studies have employed the hapten trinitrophenol (TNP) coupled to splenocytes or specific lymphocyte subsets to induce TNP-ACAID. The three features of ACAID which are central to the phenomenon are: systemic suppression of delayed-type hypersensitivity (DTH), enhanced antibody production, and normal T cell cytoxicity. We have demonstrated that suppression of DTH is: (1) mediated by a complex immunoregulatory network of T suppressor cells; (2) initiated by the elaboration of T suppressor inducer factor (TsIF) from the eye, which homes to the spleen; and (3) dependent upon the entrance of visible light into the eye. We propose to further our studies in this model system focusing on the suppression of DTH, the modulation of antibody production to TNP and the in vitro analysis of these events.
Our specific aims are: (1) the role of lymphokines/cytokines in the suppression of DTH in TNP-ACAID; (2) the in situ study of ocular immunoregulation of antibody production to TNP; (3) the in vitro study of ocular immunoregulation of antibody production to TNP.

National Institute of Health (NIH)
National Eye Institute (NEI)
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Visual Sciences A Study Section (VISA)
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Washington University
Schools of Medicine
Saint Louis
United States
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