Our goal is to investigate the biochemistry and physiological significance of the arachidonic acid cascade, particularly the lipoxygenase-leukotriene pathway, and the inositol lipid cycle in the retina. The focus is on visual cell - pigment epithelium interactions during photoreceptor renewal. In other systems, metabolites arising from receptor-stimulated turnover of phosphoinositides and from the arachidonic acid cascade are potent mediators of intracellular and intercellular signals. The proposed experiments will: 1) correlate changes in arachidonic acid metabolism in rod outer segments and pigment epithelium with particular stages (times) of photoreceptor shedding; 2) study the effect of inhibitors of leukotriene and prostaglandin synthesis on photoreceptor disk shedding in an eyecup preparation; 3) investigate the involvement of inositol polyphosphates and the inositol lipid cycle as a transmembrane signaling system for modulation of shedding and/or phagocytosis; and 4) characterize the involvement of the arachidonic acid cascade and inositol lipids in the modulation of phagocytosis in pigment epithelium cells in culture. Powerful analytical procedures, such as high performance liquid chromatography, capillary and open column gas-liquid chromatography, gas chromatography-mass spectrometry and radioimmunoassay, will be used to examine biochemical changes and to establish correlates with a histological assessment of disk shedding and phagocytosis. The results obtained will define the involvement of the arachidonic acid cascade and the inositol lipid cycle in the modulation of the interactions between visual cells and retinal pigment epithelium.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY005121-06
Application #
3259973
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1984-03-01
Project End
1992-04-30
Budget Start
1989-05-01
Budget End
1990-04-30
Support Year
6
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Louisiana State University Hsc New Orleans
Department
Type
Schools of Medicine
DUNS #
782627814
City
New Orleans
State
LA
Country
United States
Zip Code
70112
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Belayev, Ludmila; Mukherjee, Pranab K; Balaszczuk, Veronica et al. (2017) Neuroprotectin D1 upregulates Iduna expression and provides protection in cellular uncompensated oxidative stress and in experimental ischemic stroke. Cell Death Differ 24:1091-1099
Bazan, Nicolas G (2016) Molecular Principles for Decoding Homeostasis Disruptions in the Retinal Pigment Epithelium: Significance of Lipid Mediators to Retinal Degenerative Diseases. Adv Exp Med Biol 854:385-91

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