The overall objective of these studies is to quantitate the reserve capacity of the endothelial fluid pump. Clinical studies have indicated that the failure of this pump to compensate for increases in endothelial permeability, as occurs in Fuchs for example, is responsible for the corneal edema noted in these patients. The limited reserve capacity of the endothelial pump could thus be a primary factor in the development of corneal edema. These studies, aimed at quantitating alterations in endothelial pump function at the membrane level, will further our understanding of adaptive mechanisms and physiologic reserve of the endothelial pump. These studies will employ the specific binding of tritiated ouabain to endothelial Na/K ATPase to quantitate alterations in pump site density and distribution. ATPase activity will be assayed using the 32P-ATP assay. These data will be correlated to the physiologic evaluation of endothelial function (in vitro perfusions) and endothelial morphology (light histology and wide-field specular microscopy). Answers will be sought to the following questions: 1) Are changes in endothelial pump function associated with in vivo wound healing? 2) How do the density, distribution and activity of Na-K pump sites in cultured corneal endothelial cells compare to fresh tissue? And, are changes in these parameters associated with the establishment of an intact (contact inhibited) monolayer? 3) Do changes in Na-K pump and barrier functions occur in stored corneal endothelium? 4) What is the density of Na-K pump sites in other transporting ocular tissues (ciliary body and lens epithelium)?

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY005609-03
Application #
3260798
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1985-01-01
Project End
1987-12-31
Budget Start
1987-01-01
Budget End
1987-12-31
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Medical College of Wisconsin
Department
Type
Schools of Medicine
DUNS #
073134603
City
Milwaukee
State
WI
Country
United States
Zip Code
53226
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Edelhauser, H F; Geroski, D H; Woods, W D et al. (1993) Swelling in the isolated perfused cornea induced by 12(R)hydroxyeicosatetraenoic acid. Invest Ophthalmol Vis Sci 34:2953-61
Geroski, D H; Hadley, A (1992) Characterization of corneal endothelium cell cultured on microporous membrane filters. Curr Eye Res 11:61-72
Kim, E K; Geroski, D H; Holley, G P et al. (1992) Corneal endothelial cytoskeletal changes in F-actin with aging, diabetes, and after cytochalasin exposure. Am J Ophthalmol 114:329-35
Stiemke, M M; Edelhauser, H F; Geroski, D H (1991) The developing corneal endothelium: correlation of morphology, hydration and Na/K ATPase pump site density. Curr Eye Res 10:145-56
McDermott, M L; Watsky, M A; Geroski, D H et al. (1991) Human corneal storage in modified McCarey-Kaufman and K-Sol media: effect on endothelial Na+/K+ ATPase pump site density and permeability. Cornea 10:44-9
McDermott, M L; Watsky, M A; Geroski, D H et al. (1990) A method for the in vitro determination of feline corneal endothelial permeability. Curr Eye Res 9:1129-36
Geroski, D H; Edelhauser, H F (1989) Morphometric analysis of the corneal endothelium. Specular microscopy vs. alizarin red staining. Invest Ophthalmol Vis Sci 30:254-9
Macdonald, J M; Geroski, D H; Edelhauser, H F (1987) Effect of inflammation on the corneal endothelial pump and barrier. Curr Eye Res 6:1125-32
Yee, R W; Geroski, D H; Matsuda, M et al. (1985) Correlation of corneal endothelial pump site density, barrier function, and morphology in wound repair. Invest Ophthalmol Vis Sci 26:1191-201

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