The long-term goal of the proposed research is to develop an improved capability for immunodiagnosis of ocular toxocariasis. Ocular toxocariasis is a zoonosis, frequently affecting children, which may result in considerable ocular disease. It is important to accurately diagnose ocular toxocariasis when the disease presentation and medical history are typical of infection with larval Toxocara canis. Furthermore, ocular toxocariasis is a disease condition which is often considered in the differential diagnosis of other serious diseases, such as retinoblastoma. Accordingly, it is important to reliably exclude ocular toxocariasis from certain differential diagnoses. Specific goals of the proposed research include the development of improved antibody-detection assays using larval excretory-secretory antigen fractions which have been purified by physicochemical and immunochemical means. Assays will also be developed for the detection and measurement of soluble parasite antigens. Methods will be developed initially using biological fluids, ie. serum, ocular fluids and cerebrospinal fluid, obtained from experimentally infected laboratory animals. As methods are perfected, they will be adapted for use in humans using available material. Finally, in order to develop a capability for retrospective diagnosis of ocular toxocariasis, methods will be developed for detection of parasite antigen in pathologic specimens.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
7R01EY005677-04
Application #
3261029
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1987-07-01
Project End
1989-06-30
Budget Start
1987-07-01
Budget End
1988-06-30
Support Year
4
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Colorado State University-Fort Collins
Department
Type
Schools of Veterinary Medicine
DUNS #
112617480
City
Fort Collins
State
CO
Country
United States
Zip Code
80523
Parsons, J C; Coffman, R L; Grieve, R B (1993) Antibody to interleukin 5 prevents blood and tissue eosinophilia but not liver trapping in murine larval toxocariasis. Parasite Immunol 15:501-8
Parsons, J C; Grieve, R B (1990) Kinetics of liver trapping of infective larvae in murine toxocariasis. J Parasitol 76:529-36
Grieve, R B (1990) Immunologic relevance of the cuticle and epicuticle of larval Dirofilaria immitis and Toxocara canis. Acta Trop 47:399-402
Parsons, J C; Grieve, R B (1990) Effect of egg dosage and host genotype on liver trapping in murine larval toxocariasis. J Parasitol 76:53-8
Parsons, J C; Bowman, D D; Grieve, R B (1989) Pathological and haematological responses of cats experimentally infected with Toxocara canis larvae. Int J Parasitol 19:479-88
Parsons, J C; Bowman, D D; Gillette, D M et al. (1988) Disseminated granulomatous disease in a cat caused by larvae of Toxocara canis. J Comp Pathol 99:343-6
Badley, J E; Grieve, R B; Rockey, J H et al. (1987) Immune-mediated adherence of eosinophils to Toxocara canis infective larvae: the role of excretory-secretory antigens. Parasite Immunol 9:133-43
Glickman, L T; Shofer, F S (1987) Zoonotic visceral and ocular larva migrans. Vet Clin North Am Small Anim Pract 17:39-53
Bowman, D D; Mika-Grieve, M; Grieve, R B (1987) Circulating excretory-secretory antigen levels and specific antibody responses in mice infected with Toxocara canis. Am J Trop Med Hyg 36:75-82
Badley, J E; Grieve, R B; Bowman, D D et al. (1987) Analysis of Toxocara canis larval excretory-secretory antigens: physicochemical characterization and antibody recognition. J Parasitol 73:593-600

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