Embryonic development of the eye involves inductive interactions between the various components of the eye. These interactions imply the existence of pathways for communication and coordination, but the molecular mechanisms for information exchange have not as yet been elucidate. The general strategy of the proposed research is to use the alphaA-crystallin promoter to obtain lens-specific expression of growth factors that have been identified as potentially relevant for ocular development or differentiation.
The specific aims are to express proteins from the following growth factor families in the embryonic lenses of transgenic mice: 1) fibroblast growth factor (FGF) family 2) transforming growth factors and the developmental changes of the eye. Although acidic FGF and IG-I are thought to function through activation of tyrosine kinase receptors, the preliminary results in the transgenic eyes indicate that these growth factors do not cause the same changes in lenticular differentiation. In the future, theses transgenic eyes can be use to assess the divergent signal transduction pathways in order to begin to identify the critical components. The overall objective of these experiments is to gain insight into growth factor function and molecular regulation of normal development of the eye. A better molecular understanding of ocular morphogenesis will help guide the planning and implementation of strategies to help prevent or alleviate visual disorders and ocular birth defects.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
1R01EY010448-01
Application #
2164325
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1994-01-01
Project End
1996-12-31
Budget Start
1994-01-01
Budget End
1994-12-31
Support Year
1
Fiscal Year
1994
Total Cost
Indirect Cost
Name
Baylor College of Medicine
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030
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Govindarajan, Venkatesh; Overbeek, Paul A (2006) FGF9 can induce endochondral ossification in cranial mesenchyme. BMC Dev Biol 6:7
Xie, Leike; Overbeek, Paul A; Reneker, Lixing W (2006) Ras signaling is essential for lens cell proliferation and lens growth during development. Dev Biol 298:403-14
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Chen, Qin; Liang, Dongcai; Yang, Tao et al. (2004) Distinct capacities of individual E2Fs to induce cell cycle re-entry in postmitotic lens fiber cells of transgenic mice. Dev Neurosci 26:435-45
Reneker, Lixing W; Chen, Qin; Bloch, Amy et al. (2004) Chick delta1-crystallin enhancer influences mouse alphaA-crystallin promoter activity in transgenic mice. Invest Ophthalmol Vis Sci 45:4083-90

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