The long-term objective of the proposed research is to understand the cellular mechanisms that underlie vision. The premise underlying this application is that the remarkable adaptability of vision is based on the plasticity of intracellular signaling pathways in retinal neurons. The focus of the work will be to study the mechanisms that regulate the intracellular Ca2+ concentration and transmitter release in retinal rod and cone photoreceptor neurons. The proposed experiments combine electrophysiological, optical and immunocytochemical methods. Results obtained in these studies will help us select cellular targets for therapeutic interventions during retinal disease and blindness.
Three specific aims are proposed: (1) to characterize Ca2+ regulation in photoreceptor inner segments; (2) to identify differences in Ca2+ homeostasis between rod and cone photoreceptor inner segments; and (3) to determine the relationship between [Ca2+]і and exocytosis in photoreceptors.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY013870-02
Application #
6620782
Study Section
Visual Sciences C Study Section (VISC)
Program Officer
Mariani, Andrew P
Project Start
2002-03-01
Project End
2006-02-28
Budget Start
2003-03-01
Budget End
2004-02-28
Support Year
2
Fiscal Year
2003
Total Cost
$226,750
Indirect Cost
Name
University of California San Francisco
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
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Križaj, David (2016) Polymodal Sensory Integration in Retinal Ganglion Cells. Adv Exp Med Biol 854:693-8
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Križaj, David; Ryskamp, Daniel A; Tian, Ning et al. (2014) From mechanosensitivity to inflammatory responses: new players in the pathology of glaucoma. Curr Eye Res 39:105-19
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