The interplexiform cell has been the last major class of retinal neurons to be discovered. It provides long distance feedback from the inner retina to the outer retina, yet little is known about the function of this class of neuron. The interplexiform cell is the only neuron that provides glycinergic signals to the distal retina in amphibia. This provides an opportunity to explore its function. In this proposal, I will investigate how glycinergic interplexiform cells regulate the physiology of the distal retina. The overall hypothesis is that glycinergic interplexiform cells enhance the output of photoreceptors and the level of enhancement increases as the retina progresses from a dark to a light adapted state.
The first aim will be to determine the mechanisms by which the glycinergic interplexiform cells increase photoreceptor transmitter release.
The second aim i s to evaluate the direct effect of glycinergic interplexiform cell on horizontal cells, and to discern the mechanism of action. The third specific aim is to contrast the physiology of the glycinergic interplexiform cell in the dark and the light adapted retina. The application will place an emphasis on how glycinergic interplexiform cells affect neurotransmitter release in rods and cones, and on the fundamental mechanisms of the glycinergic interplexiform cell in dark and light adapted states in terms of the effects on photoreceptor transmitter release and horizontal cell depolarization. This analysis will serve as a contrast to the dopamine system, another mediator of light adaptation. The results of this study should provide an improved appreciation of the role of the interplexiform cell and should also expand knowledge of the molecular events that occur during light adaptation.

National Institute of Health (NIH)
National Eye Institute (NEI)
Research Project (R01)
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Special Emphasis Panel (ZRG1-VISC (01))
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Mariani, Andrew P
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Florida Atlantic University
Other Domestic Higher Education
Boca Raton
United States
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Shen, Wen; Nan, Changlong; Nelson, Peter T et al. (2017) GABAB receptor attenuation of GABAA currents in neurons of the mammalian central nervous system. Physiol Rep 5:
Yang, Jinnan; Nan, ChangLong; Ripps, Harris et al. (2015) Destructive Changes in the Neuronal Structure of the FVB/N Mouse Retina. PLoS One 10:e0129719
Jiang, Zheng; Yang, Jinnan; Purpura, Lauren A et al. (2014) Glycinergic feedback enhances synaptic gain in the distal retina. J Physiol 592:1479-92
Jiang, Zheng; Bulley, Simon; Guzzone, Joseph et al. (2013) The modulatory role of taurine in retinal ganglion cells. Adv Exp Med Biol 775:53-68
Anastassov, Ivan; Shen, Wen; Ripps, Harris et al. (2013) Zinc modulation of calcium activity at the photoreceptor terminal: a calcium imaging study. Exp Eye Res 112:37-44
Shen, Wen; Purpura, Lauren A; Li, Baoqin et al. (2013) Regulation of synaptic transmission at the photoreceptor terminal: a novel role for the cation-chloride co-transporter NKCC1. J Physiol 591:133-47
Rowan, Matthew J M; Bulley, Simon; Purpura, Lauren A et al. (2013) Taurine regulation of voltage-gated channels in retinal neurons. Adv Exp Med Biol 775:85-99
Bulley, Simon; Liu, Yufei; Ripps, Harris et al. (2013) Taurine activates delayed rectifier Kv channels via a metabotropic pathway in retinal neurons. J Physiol 591:123-32
Ripps, Harris; Shen, Wen (2012) Review: taurine: a ""very essential"" amino acid. Mol Vis 18:2673-86
Jiang, Zheng; Shen, Wen (2010) Role of neurotransmitter receptors in mediating light-evoked responses in retinal interplexiform cells. J Neurophysiol 103:924-33

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