Over the next 5 years, the main objective of this project will be to analyze visually evoked synaptic activity in tectal neurons of Xenopus tadpoles. In particular, the temporal and spatial patterns of active regions on the dendritic trees will be examined during development in general and also during periods of induced changes in synaptic strength. To this end patterned visual stimulation and multiple patch-clamp recordings of tectal cells will be combined with simultaneous multi-photon laser scanning calcium imaging. Patch clamp recordings will provide the amplitude and the fast temporal dynamics of synaptic responses and two-photon calcium imaging will reveal the spatial pattern in three dimensions. Synapses will be activated by direct electrical stimulation of retinal ganglion cells as well as by visual images presented to the retina. To induce changes in synaptic connectivity several electrical and visual stimulation paradigms will be used. These experiments will provide new information about dendritic integration of complex physiological inputs and about the development of receptive fields in the vertebrate visual system. ? ?

National Institute of Health (NIH)
National Eye Institute (NEI)
Research Project (R01)
Project #
Application #
Study Section
Central Visual Processing Study Section (CVP)
Program Officer
Oberdorfer, Michael
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Harvard University
Schools of Arts and Sciences
United States
Zip Code
Lefort, Sandrine; Gray, Annette C; Turrigiano, Gina G (2013) Long-term inhibitory plasticity in visual cortical layer 4 switches sign at the opening of the critical period. Proc Natl Acad Sci U S A 110:E4540-7
Ahrens, Misha B; Li, Jennifer M; Orger, Michael B et al. (2012) Brain-wide neuronal dynamics during motor adaptation in zebrafish. Nature 485:471-7
Valente, Andre; Huang, Kuo-Hua; Portugues, Ruben et al. (2012) Ontogeny of classical and operant learning behaviors in zebrafish. Learn Mem 19:170-7
Naumann, Eva A; Kampff, Adam R; Prober, David A et al. (2010) Monitoring neural activity with bioluminescence during natural behavior. Nat Neurosci 13:513-20
Bollmann, Johann H; Engert, Florian (2009) Subcellular topography of visually driven dendritic activity in the vertebrate visual system. Neuron 61:895-905
Bracken, Bethany K; Turrigiano, Gina G (2009) Experience-dependent regulation of TrkB isoforms in rodent visual cortex. Dev Neurobiol 69:267-78
Maffei, Arianna; Turrigiano, Gina G (2008) Multiple modes of network homeostasis in visual cortical layer 2/3. J Neurosci 28:4377-84
Ramdya, Pavan; Engert, Florian (2008) Emergence of binocular functional properties in a monocular neural circuit. Nat Neurosci 11:1083-90
Orger, Michael B; Kampff, Adam R; Severi, Kristen E et al. (2008) Control of visually guided behavior by distinct populations of spinal projection neurons. Nat Neurosci 11:327-33
Douglass, Adam D; Kraves, Sebastian; Deisseroth, Karl et al. (2008) Escape behavior elicited by single, channelrhodopsin-2-evoked spikes in zebrafish somatosensory neurons. Curr Biol 18:1133-7

Showing the most recent 10 out of 11 publications