In the past decade, several CNS-based neural prosthetics have achieved remarkable clinical outcomes. For example, deep brain stimulation (DBS) is now routinely used to treat Parkinsonian tremors and cochlear prosthetics stimulate the auditory nerve to restore high levels of hearing to the profoundly deaf. However, not all devices have achieved the same level of success. For example, retinal prosthetics do not reliably elicit complex (or even simple) spatial percepts even though individual electrodes can consistently elicit focal percepts (phosphenes). Even the more successful applications can sometimes be inconsistent and have created unwanted side effects. To improve the outcomes associated with retinal (and other neural) prosthetics, we are studying the fundamental interactions between electric stimulation and targeted neurons using a combination of electrophysiology, anatomy and computational modeling. In retinal neurons, we found that the lowest thresholds in response to electric stimulation occur when the stimulating electrode is positioned over the proximal portion of the axon (near the soma). This region of low threshold is precise aligned with a dense band of voltage-gated sodium channels identified immunochemically. Different types of retinal neurons have different bands (lengths and locations) as well as different absolute (lowest) thresholds. This implies that band differences contribute to threshold differences and we propose to investigate the details by which this occurs. It is likely that the band is also the site in which spikes are initiated but this has not yet been confirmed;here, we propose several experiments to determine whether the band is in fact the site. Knowledge of the site of spike initiation enables us to systematically study how changes to the applied electric field (arising from the stimulus pulse) alter the neural response. Our preliminary data suggests that analogous to stimulation of axons, the second spatial derivative of the induced voltage profile along the band is a good predictor of pulse efficacy. In the temporal domain, we want to determine which stimulus profile is most effective for activating different elements within the neuron. We are also exploring whether specific classes or sub-classes of neurons respond preferentially to different stimulus frequencies. Our hope is that by understanding the basic elements of the response mechanism, including the cause of response differences between different types of neurons, we can develop more effective stimulation methods that will lead to better clinical outcomes.

Public Health Relevance

Despite remarkable progress with some CNS-based neural prosthetics, devices that target other regions of the CNS have not yet achieved satisfactory clinical results. As a step towards developing more effective stimulation methods for retinal prosthetics, we are studying the fundamental interactions between electric stimulation and targeted retinal neurons. Because the knowledge acquired in these studies pertains to general activation mechanisms, it is likely to be applicable to both the retinal prosthetic as well as a wide range of other CNS- based devices.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY019967-02
Application #
8007366
Study Section
Special Emphasis Panel (ZRG1-ETTN-E (92))
Program Officer
Greenwell, Thomas
Project Start
2010-01-01
Project End
2014-12-31
Budget Start
2011-01-01
Budget End
2011-12-31
Support Year
2
Fiscal Year
2011
Total Cost
$373,495
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199
Twyford, Perry; Fried, Shelley (2016) The Retinal Response to Sinusoidal Electrical Stimulation. IEEE Trans Neural Syst Rehabil Eng 24:413-23
Im, Maesoon; Fried, Shelley I (2016) Directionally selective retinal ganglion cells suppress luminance responses during natural viewing. Sci Rep 6:35708
Im, Maesoon; Fried, Shelley I (2016) Temporal properties of network-mediated responses to repetitive stimuli are dependent upon retinal ganglion cell type. J Neural Eng 13:025002
Im, Maesoon; Fried, Shelley I (2015) Indirect activation elicits strong correlations between light and electrical responses in ON but not OFF retinal ganglion cells. J Physiol 593:3577-96
Lee, Seung Woo; Fried, Shelley I (2015) Suppression of subthalamic nucleus activity by micromagnetic stimulation. IEEE Trans Neural Syst Rehabil Eng 23:116-27
Guo, Tianruo; Lovell, Nigel H; Tsai, David et al. (2014) Selective activation of ON and OFF retinal ganglion cells to high-frequency electrical stimulation: a computational modeling study. Conf Proc IEEE Eng Med Biol Soc 2014:6108-11
Werginz, P; Fried, S I; Rattay, F (2014) Influence of the sodium channel band on retinal ganglion cell excitation during electric stimulation--a modeling study. Neuroscience 266:162-77
Lee, Seung Woo; Fried, Shelley I (2014) The response of L5 pyramidal neurons of the PFC to magnetic stimulation from a micro-coil. Conf Proc IEEE Eng Med Biol Soc 2014:6125-8
Twyford, Perry; Cai, Changsi; Fried, Shelley (2014) Differential responses to high-frequency electrical stimulation in ON and OFF retinal ganglion cells. J Neural Eng 11:025001
Fang, Yuan; Cho, Kin-Sang; Tchedre, Kissaou et al. (2013) Ephrin-A3 suppresses Wnt signaling to control retinal stem cell potency. Stem Cells 31:349-59

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