Abstract

This application is to request an amendment for existing RO1 to purchase replacement equipment including freezer, BL2 hood, Flow cytometer and respective software. In additions the supplement seeks funds to compensate for sustained losses in animal costs, effort, reagents during the COVID19 lockdown.

Public Health Relevance

The funded application evaluates the impact of different commensal species or communities on promoting resistance to keratitis and the mechanisms responsible for protection. This amendment is to seek additional funds to recover from the interruptions due to COVID19 lockdown of the laboratory and to request funds to purchase limited vital equipment needed to replace nonfunctional equipment.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
3R01EY022054-09S1
Application #
10226649
Study Section
Program Officer
Mckie, George Ann
Project Start
2012-08-01
Project End
2021-07-31
Budget Start
2020-09-01
Budget End
2021-07-31
Support Year
9
Fiscal Year
2020
Total Cost
Indirect Cost

  Institution

Name
Brigham and Women's Hospital
Department
Type
DUNS #
030811269
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Kugadas, Abirami; Wright, Quentin; Geddes-McAlister, Jennifer et al. (2017) Role of Microbiota in Strengthening Ocular Mucosal Barrier Function Through Secretory IgA. Invest Ophthalmol Vis Sci 58:4593-4600
St Leger, Anthony J; Desai, Jigar V; Drummond, Rebecca A et al. (2017) An Ocular Commensal Protects against Corneal Infection by Driving an Interleukin-17 Response from Mucosal ?? T Cells. Immunity 47:148-158.e5
Pandya, Hardik J; Kanakasabapathy, Manoj Kumar; Verma, Saloni et al. (2017) Label-free electrical sensing of bacteria in eye wash samples: A step towards point-of-care detection of pathogens in patients with infectious keratitis. Biosens Bioelectron 91:32-39
Christiansen, Stig Hill; Murphy, Ronan A; Juul-Madsen, Kristian et al. (2017) The Immunomodulatory Drug Glatiramer Acetate is Also an Effective Antimicrobial Agent that Kills Gram-negative Bacteria. Sci Rep 7:15653
Roux, Damien; Weatherholt, Molly; Clark, Bradley et al. (2017) Immune Recognition of the Epidemic Cystic Fibrosis Pathogen Burkholderia dolosa. Infect Immun 85:
Kugadas, Abirami; Gadjeva, Mihaela (2016) Impact of Microbiome on Ocular Health. Ocul Surf 14:342-9
Chen, Li; Zhou, Xia; Fan, Lucy X et al. (2015) Macrophage migration inhibitory factor promotes cyst growth in polycystic kidney disease. J Clin Invest 125:2399-412
Terzulli, Marielle; Contreras-Ruiz, Laura; Ruiz, Laura Contreras et al. (2015) TSP-1 Deficiency Alters Ocular Microbiota: Implications for Sjögren's Syndrome Pathogenesis. J Ocul Pharmacol Ther 31:413-8
Gauguet, Stefanie; D'Ortona, Samantha; Ahnger-Pier, Kathryn et al. (2015) Intestinal Microbiota of Mice Influences Resistance to Staphylococcus aureus Pneumonia. Infect Immun 83:4003-14
Shan, Qiang; Dwyer, Markryan; Rahman, Samir et al. (2014) Distinct susceptibilities of corneal Pseudomonas aeruginosa clinical isolates to neutrophil extracellular trap-mediated immunity. Infect Immun 82:4135-43

Showing the most recent 10 out of 13 publications