Diabetic retinopathy is a complication of diabetes that induces visual impairment, and is a leading cause of blindness. According to the U.S. Centers for Disease Control and Prevention, 45% of patients diagnosed with diabetes develop diabetic retinopathy. Altered retinal blood flow is an early consequence of the disease, and may contribute to vascular damage that occurs as the disease progresses. Thus, understanding the pathogenesis of vascular dysfunction is essential to preventing later stages of diabetic retinopathy. From its onset, diabetic retinopathy is characterized by an inability of the retinal blood supply to match neuronal demand for uninterrupted delivery of oxygen and nutrients. In the retina, most of these interactions take place at the level of capillaries, where pericytes, the only contractile cells at this level, adjust capillary diameter in response to neuronal signals. Th neurotransmitter acetylcholine, released by cholinergic neurons, induces pericyte-mediated vasodilation. Both pericytes and cholinergic neurons are structurally compromised in diabetic retinopathy. It is unclear, however, whether this translates into impaired vasomotor function. To address this, in Aim 1 we will test the hypothesis that decline in vasomotor ability of pericytes and changes in their gap junction coupling mediate impaired vasomotor control in diabetic retina. We will (1a) measure pericyte activity in response to electrical and chemical stimulation, then (1b) determine whether decreased gap junction coupling reduces pericyte activity propagation. This is relevant, since individual pericytes control local capillary diameter, while signaling between pericytes via gap junctions allows for the spatial distribution of vasomotor control.
In Aim 2, we will test the hypothesis that reduced vascular sensitivity to cholinergic signaling and a decline in cholinergic amacrine cell function both impair vasomotor activity. First we will (2a) use cholinergic agonists and antagonists to determine whether the efficacy of cholinergic regulation of capillary diameter is compromised in diabetic retina. Then, (2b) we will use an optogenetic approach to directly activate cholinergic amacrine cells, to determine whether they are functionally compromised. Altered retinal blood flow is a common characteristic of patients with early stages of diabetic retinopathy, and likely contributes to latr complications of the disease. Functional compromises to the cells responsible for regulating blood flow in the retina may account for this vasomotor deficit. Understanding the mechanisms of vasomotor dysfunction could reveal novel therapeutic targets, and provide additional approaches for treating diabetic retinopathy.

Public Health Relevance

Diabetic retinopathy is a complication of diabetes, and is an increasingly common cause of visual impairment. Nearly half of patients diagnosed with diabetes - both type 1 and type 2 - suffer from diabetic retinopathy. The retina has one of the highest metabolic demands in the human body, and requires neuronal control of blood flow to maintain uninterrupted delivery of oxygen and other nutrients. From its onset, diabetic retinopathy is characterized by the inability of blood supply to match neuronal demand. In the retina, most of these interactions take place at the level of capillaries, where specialized vascular cells adjust blood flow in response to neuronal signals. The goal of this project is to determine how the altered interactions between these vascular cells and neurons lead to disrupted blood flow control in diabetes. Altered retinal blood flow is an early consequence of the disease, and may contribute to the further complications in vascular health that occur as the disease progresses. Thus, determining deficiencies in blood flow control will help prevent and treat complications of diabetic retinopathy.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY026576-04
Application #
9684624
Study Section
Diseases and Pathophysiology of the Visual System Study Section (DPVS)
Program Officer
Shen, Grace L
Project Start
2016-04-01
Project End
2021-03-31
Budget Start
2019-04-01
Budget End
2020-03-31
Support Year
4
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Winifred Masterson Burke Med Research Institute
Department
Type
DUNS #
780676131
City
White Plains
State
NY
Country
United States
Zip Code
10605
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