The development of mass spectrometric methods for the determination of the primary structure of proteins will be continued and applied to new proteins. The sensitivity of the reductive GCMS method for peptide sequencing will be improved by carrying out the derivatization in the gas phase. Fast-atom bombardment and tandem mass spectrometry will be used to determine peptide sequences. Laser induced fragmentation will be investigated for this purpose. These methodologies will be used to determine the structure of a series of thioredoxins, ubiquitous enzymes present in all organisms from bacteria to man; of a viral coat protein and other proteins. Specific enzymatic and chemical peptide bond cleavage methods will be explored for their specific utility in these studies.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM005472-32
Application #
3267886
Study Section
Special Emphasis Panel (BET)
Project Start
1977-01-01
Project End
1990-12-31
Budget Start
1990-01-01
Budget End
1990-12-31
Support Year
32
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139
Biemann, Klaus (2015) Structure Determination of Natural Products by Mass Spectrometry. Annu Rev Anal Chem (Palo Alto Calif) 8:1-19
Biemann, Klaus (2014) Laying the groundwork for proteomics: mass spectrometry from 1958 to 1988. J Proteomics 107:62-70