Abstract

The long-term objectives of the proposed research program has as its aim the study of various aspects of human plasma proteins and glycoproteins. The following two areas of investigation were selected as specific goals: 1. Isolation and chemical and physicochemical characterization of biologically important proteins: The plasma gonadotropins, serine-threonine-rich glycoproteins, and an immunoregulatory peptide. It should be noted that the study of these proteins is pertinent to important health-related problems. The immunoregulatory peptide is directly related to organ transplantation and auto-immune diseases. In addition, it will be attempted to make a significant contribution toward the elucidation of the etiology of the Moyamoya disease. 2. Determination of the complete structure of alpha 1-acid glycoprotein, a normal human plasma protein, whose amino acid sequence has recently been established in the applicant's laboratory.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM010374-25
Application #
3268102
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1977-12-01
Project End
1988-11-30
Budget Start
1986-12-01
Budget End
1988-11-30
Support Year
25
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Boston University
Department
Type
Schools of Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02118

  Publications

Binette, J P; Burgi, W; Ohishi, H et al. (1994) The glycosaminoglycan composition of human tracheas and the changes observed during aging and in disease. Clin Chim Acta 225:179-85
Liu, H M; Lei, H Y; Schmid, K (1993) Alpha 1-acid glycoprotein and peripheral nerve injury, studied with a wound chamber. Lab Invest 68:577-83
Schmid, K; Hediger, M A; Brossmer, R et al. (1992) Amino acid sequence of human plasma galactoglycoprotein: identity with the extracellular region of CD43 (sialophorin). Proc Natl Acad Sci U S A 89:663-7
Watzlawick, H; Walsh, M T; Yoshioka, Y et al. (1992) Structure of the N- and O-glycans of the A-chain of human plasma alpha 2HS-glycoprotein as deduced from the chemical compositions of the derivatives prepared by stepwise degradation with exoglycosidases. Biochemistry 31:12198-203
Grundboeck-Jusco, J; Binette, J P; Kimura, A et al. (1992) The glycosaminoglycan composition of the lung with acute and chronic pathology and in senescence. Clin Chim Acta 208:77-84
Mikami-Takei, K; Kosakai, M; Isemura, M et al. (1991) Fractionation of jelly substance of the sea urchin egg and biological activities to induce acrosome reaction and agglutination of spermatozoa. Exp Cell Res 192:82-6
Ohishi, H; Skinner, M; Sato-Araki, N et al. (1990) Glycosaminoglycans of the hemodialysis-associated carpal synovial amyloid and of amyloid-rich tissues and fibrils of heart, liver, and spleen. Clin Chem 36:88-91
Walsh, M T; Watzlawick, H; Putnam, F W et al. (1990) Effect of the carbohydrate moiety on the secondary structure of beta 2-glycoprotein. I. Implications for the biosynthesis and folding of glycoproteins. Biochemistry 29:6250-7
Burgi, W; Simonen, S; Baudner, S et al. (1989) Unusually high concentrations of Zn alpha 2-glycoprotein and the lack of alpha 2HS-glycoprotein in human ejaculates. Clin Chem 35:1649-50
Araki, T; Yoshioka, Y; Schmid, K (1989) The position of the disulfide bonds in human plasma alpha 2 HS-glycoprotein and the repeating double disulfide bonds in the domain structure. Biochim Biophys Acta 994:195-9

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