Abstract

Our work is on gene expression in eukaryotic organisms and the mechanisms which control this expression. We are attacking this in 4 separate projects. Two projects deal with the mechanism of the termination of transcription in eukaryotes. We are determining the nucleotide sequence of the 3' end of the mRNA for the Z gene of Drosophila in the region just preceding the poly(A) tract to try to identify a termination or processing signal in the sequence. We are also trying to isolate a factor like rho from yeast and other eukaryotes. A third project deals with the control of actin synthesis in the sea urchin embryo and the time of appearance of different actin mRNAs during cleavage. We now have a variety of cloned sea urchin actin genes to be used as probes for determining actin mRNAs. We still need to identify these different actin genes. A fourth project deals with the same controls in the case of tubulin synthesis in the cleaving sea urchin embryo. For this project we are attempting to isolate tubulin genes from the library of sea urchin genes cloned in lambda charon 4A.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM010791-23
Application #
3268136
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1976-02-01
Project End
1986-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
23
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Cornell University
Department
Type
Schools of Arts and Sciences
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850

  Publications

Lu, J; Lee, W; Jiang, C et al. (1994) Start site selection by Sp1 in the TATA-less human Ha-ras promoter. J Biol Chem 269:5391-402
Lu, J X (1992) Magnesium chelation inactivates beta-galactosidase in -20 degrees C storage. Biotechniques 12:177-81
Lu, J; Jiang, C (1992) Detergents inhibit chloramphenicol acetyl transferase. Biotechniques 12:643-4
Chung, Y T; Keller, E B (1991) The TATA-dependent and TATA-independent promoters of the Drosophila melanogaster actin 5C-encoding gene. Gene 106:237-41
Lee, W; Keller, E B (1991) Regulatory elements mediating transcription of the human Ha-ras gene. J Mol Biol 220:599-611
Chung, Y T; Keller, E B (1990) Positive and negative regulatory elements mediating transcription from the Drosophila melanogaster actin 5C distal promoter. Mol Cell Biol 10:6172-80
Chung, Y T; Keller, E B (1990) Regulatory elements mediating transcription from the Drosophila melanogaster actin 5C proximal promoter. Mol Cell Biol 10:206-16
Kindle, K L (1990) High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A 87:1228-32
Kindle, K L; Schnell, R A; Fernandez, E et al. (1989) Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase. J Cell Biol 109:2589-601
Gunderson, J H; Elwood, H; Ingold, A et al. (1987) Phylogenetic relationships between chlorophytes, chrysophytes, and oomycetes. Proc Natl Acad Sci U S A 84:5823-7

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