New molecular spectroscopic approaches will be developed for the selective, sensitive, precise measurement of drugs (including pesticides and polycyclic aromatic hydrocarbons) in pharmaceutical products, biological materials, and other types of samples. The new approaches involve a broad range of spectroscopic methods: including laser excited luminescence, photoacoustic, and photoionization detection of analytes separated by liquid chromatography; laser excited photothermal spectroscopy of molecules in the gas, liquid, and solid phases, particularly gas chromatography, liquid chromatography, and thin layer chromatography; evaluation of aerosols as an intermediate physical state with laser excited luminescence, photoacoustic and photoionization detection; site selection luminescence spectrometry based upon 2 photon-excitation of glasses, of crystalline matrices, of species on solid substrates, and of polymer films including the benefit of fluorescence hole burning; sequential excitation of gaseous molecules with lasers and fluorescence or ionization detection for high spectral selectivity; surface-enhanced Raman spectrometry of molecules in capillaries and on fiber optics coated with metal films and in metal hydrosols; room temperature phosphorimetry including mechanistic studies, new solid substrates based on polymer films, porous vycor and chemically modified surfaces, magnetic field effects, and the complimentary nature of room temperature delayed fluorescence; luminescence of molecules in organized media, including time resolved phosphorimetry, thin films, and micro-capillary cells; synchronous luminescence including time resolved phosphorimetry, HPLC detection, and fingerprinting of samples; measurement of analytical figures of merit of drugs, pesticides and polycyclic aromatic hydrocarbons by a variety of spectroscopic methods to provide a data base; and methods based upon the use of charge coupled devices.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM011373-27
Application #
3268258
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1977-09-01
Project End
1991-08-31
Budget Start
1989-09-01
Budget End
1990-08-31
Support Year
27
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of Florida
Department
Type
Schools of Arts and Sciences
DUNS #
073130411
City
Gainesville
State
FL
Country
United States
Zip Code
32611
Sutherland, W S; Laserna, J J; Angebranndt, M J et al. (1990) Surface-enhanced Raman analysis of sulfa drugs on colloidal silver dispersion. Anal Chem 62:689-93
Berthod, A; Glick, M; Winefordner, J D (1990) Sensitive, indirect photometric detector for high-performance liquid chromatography using a light-emitting diode. J Chromatogr 502:305-15
Campiglia, A D; Berthod, A; Winefordner, J D (1990) Solid-surface room-temperature phosphorescence detection for high-performance liquid chromatography. J Chromatogr 508:37-49
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Glick, M R; Winefordner, J D (1988) Development of a solid-substrate room-temperature luminescence immunoassay. Anal Chem 60:1982-4
Ramis Ramos, G; Garcia Alvarez-Coque, M C; O'Reilly, A M et al. (1988) Paper substrate room-temperature phosphorimetry of polyaromatic hydrocarbons enhanced by surface-active agents. Anal Chem 60:416-20
Tanabe, K; Glick, M; Smith, B et al. (1987) Development of sensitive multiwavelength fluorescence detector system for high-performance liquid chromatography. Anal Chem 59:1125-9

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