Abstract

Fluorescence activated cell sorting and analysis are widely used in clinical and biological studies to analyze and sort cells or other particles at rates of several thousand per second on the basis of fluorescence, light scattering or other optical properties. The prototypes of most instruments now used for these purposes as well as many biological and clinical applications of these instruments were developed under this grant. We now plan to investigate the use of ultrasound as a novel means of cell analysis in flow. If this proves feasible, we will incorporate acoustic detection in a sorter and correlate ultrasound measurements on different types of cells with the other measurement parameters available in flow cytometry. For our FACS instruments, we also plan to increase rates of sorting and analysis, improve efficiency of discriminating and sorting very rare cells, extend the range of optical scatter and infrared measurements, and develop better means of data management, analysis and display using a super-mini computer (VAX 11/780) interfaced with the FACS. Biological and clinical applications projects serving as interactive test systems for these developments include identifying small functional subsets of lymphoid cells, selecting hybridoma switch variants producing new types of immunoglobulins, cloning lymphocyte differentiation antigen genes (e.g. Leu-1 and Leu-2) via DNA transformation, and monitoring passage of Rh+ red cells from fetus to mother during pregnancy.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM017367-16
Application #
3269085
Study Section
(SSS)
Project Start
1976-01-01
Project End
1986-12-31
Budget Start
1985-01-01
Budget End
1985-12-31
Support Year
16
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Stanford University
Department
Type
Schools of Medicine
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Panka, D J; Mudgett-Hunter, M; Parks, D R et al. (1988) Variable region framework differences result in decreased or increased affinity of variant anti-digoxin antibodies. Proc Natl Acad Sci U S A 85:3080-4
Hagiwara, H; Huang, H J; Arai, N et al. (1987) Interleukin 1 modulates messenger RNA levels of lymphokines and of other molecules associated with T cell activation in the T cell lymphoma LBRM33-1A5. J Immunol 138:2514-9
Huang, H J; Jones, N H; Strominger, J L et al. (1987) Molecular cloning of Ly-1, a membrane glycoprotein of mouse T lymphocytes and a subset of B cells: molecular homology to its human counterpart Leu-1/T1 (CD5). Proc Natl Acad Sci U S A 84:204-8
Nakauchi, H; Tagawa, M; Nolan, G P et al. (1987) Isolation and characterization of the gene for the murine T cell differentiation antigen and immunoglobulin-related molecule, Lyt-2. Nucleic Acids Res 15:4337-47
Alberti, S; Parks, D R; Herzenberg, L A (1987) A single laser method for subtraction of cell autofluorescence in flow cytometry. Cytometry 8:114-9
Hardy, R R; Dangl, J L; Hayakawa, K et al. (1986) Frequent lambda light chain gene rearrangement and expression in a Ly-1 B lymphoma with a productive kappa chain allele. Proc Natl Acad Sci U S A 83:1438-42
Jones, N H; Clabby, M L; Dialynas, D P et al. (1986) Isolation of complementary DNA clones encoding the human lymphocyte glycoprotein T1/Leu-1. Nature 323:346-9
Kipps, T J; Herzenberg, L A (1986) Homologous chromosome recombination generating immunoglobulin allotype and isotype switch variants. EMBO J 5:263-8
Herzenberg, L A; Stall, A M; Lalor, P A et al. (1986) The Ly-1 B cell lineage. Immunol Rev 93:81-102
Tagawa, M; Nakauchi, H; Herzenberg, L A et al. (1986) Formal proof that different-size Lyt-2 polypeptides arise from differential splicing and post-transcriptional regulation. Proc Natl Acad Sci U S A 83:3422-6

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