1) The biochemical characterization of selected respiration-deficient Chinese hamster cell mutants will be continued. 2) We hope to complete the mapping of the gene of a component of succinate dehydrogenase on human chromosome #1. 3) Oligomycin resistance in hamster cells is being studied to determine if it is determined by a nuclear or cytoplasmic gene. 4) Several temperature-sensitive hamster mutants with a defect in G1 of the cell cycle are being studied, with particular emphasis on the inducibility at the nonpermissive temperature of the enzymes ornithine decarboxylase, SAM decarboxylase, thymidine kinase, and ribonucleotide reductase. 5) Variants resistant to the analogue alpha-methylornithine are being selected and studied.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM018835-15
Application #
3269403
Study Section
Mammalian Genetics Study Section (MGN)
Project Start
1977-09-01
Project End
1987-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
15
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of California San Diego
Department
Type
Schools of Arts and Sciences
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Pilz, R B; Berjis, M; Idriss, S D et al. (1994) Isolation and characterization of HL-60 cells resistant to nitroprusside-induced differentiation. J Biol Chem 269:32155-61