Abstract

We have shown that very slow motions in water-biopolymer solutions can be studied using NMR techniques, particularly on and off resonance T10 measurements. We propose to use these methods to examine proton and deuterium relaxation in concentrated polypeptide and protein systems. We also describe calculational procedures to study protein structure and to make use of experimental data to define the conformations of macromolecules in solution.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM019267-14
Application #
3269587
Study Section
Biophysics and Biophysical Chemistry B Study Section (BBCB)
Project Start
1977-12-01
Project End
1986-01-31
Budget Start
1985-02-01
Budget End
1986-01-31
Support Year
14
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
Schools of Pharmacy
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Guiles, R D; Basus, V J; Sarma, S et al. (1993) Novel heteronuclear methods of assignment transfer from a diamagnetic to a paramagnetic protein: application to rat cytochrome b5. Biochemistry 32:8329-40
Kosen, P A; Marks, C B; Falick, A M et al. (1992) Disulfide bond-coupled folding of bovine pancreatic trypsin inhibitor derivatives missing one or two disulfide bonds. Biochemistry 31:5705-17
Guiles, R D; Basus, V J; Kuntz, I D et al. (1992) Sequence-specific 1H and 15N resonance assignments for both equilibrium forms of the soluble heme binding domain of rat ferrocytochrome b5. Biochemistry 31:11365-75
Hurle, M R; Eads, C D; Pearlman, D A et al. (1992) Comparison of solution structures of mutant bovine pancreatic trypsin inhibitor proteins using two-dimensional nuclear magnetic resonance. Protein Sci 1:91-106
Hurle, M R; Anderson, S; Kuntz, I D (1991) Confirmation of the predicted source of a slow folding reaction: proline 8 of bovine pancreatic trypsin inhibitor. Protein Eng 4:451-5
Altman, J D; Henner, D; Nilsson, B et al. (1991) Intracellular expression of BPTI fusion proteins and single column cleavage/affinity purification by chymotrypsin. Protein Eng 4:593-600
Hurle, M R; Marks, C B; Kosen, P A et al. (1990) Denaturant-dependent folding of bovine pancreatic trypsin inhibitor mutants with two intact disulfide bonds. Biochemistry 29:4410-9
Babbitt, P C; West, B L; Buechter, D D et al. (1990) Removal of a proteolytic activity associated with aggregates formed from expression of creatine kinase in Escherichia coli leads to improved recovery of active enzyme. Biotechnology (N Y) 8:945-9
Bradley, E K; Thomason, J F; Cohen, F E et al. (1990) Studies of synthetic helical peptides using circular dichroism and nuclear magnetic resonance. J Mol Biol 215:607-22
Guiles, R D; Altman, J; Kuntz, I D et al. (1990) Structural studies of cytochrome b5: complete sequence-specific resonance assignments for the trypsin-solubilized microsomal ferrocytochrome b5 obtained from pig and calf. Biochemistry 29:1276-89

Showing the most recent 10 out of 18 publications