Abstract

Nuclear magnetic resonance is being applied to the study of transfer RNA, and of related proteins. Current emphasis is on identification, structure, and dynamic behavior of tRNA. Identification and structure are being approached mainly by nuclear Overhauser effect, as well as studies of carefully chosen different tRNA species. Dynamic behavior is being inferred mainly from studies of solvent exchange rates, measured by line broadening, by relaxation-time measurement, and by rapid change of solvent from H2O to D2O.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM020168-13
Application #
3269919
Study Section
Biophysics and Biophysical Chemistry A Study Section (BBCA)
Project Start
1976-06-01
Project End
1986-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
13
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Brandeis University
Department
Type
Schools of Arts and Sciences
DUNS #
616845814
City
Waltham
State
MA
Country
United States
Zip Code

  Publications

Choi, B S; Redfield, A G (1992) NMR study of nitrogen-15-labeled Escherichia coli valine transfer RNA. Biochemistry 31:12799-802
Lowry, D F; Ahmadian, M R; Redfield, A G et al. (1992) NMR study of the phosphate-binding loops of Thermus thermophilus elongation factor Tu. Biochemistry 31:2977-82
Ye, X M; Pochapsky, T C; Pochapsky, S S (1992) 1H NMR sequential assignments and identification of secondary structural elements in oxidized putidaredoxin, an electron-transfer protein from Pseudomonas. Biochemistry 31:1961-8
Miller, A F; Papastavros, M Z; Redfield, A G (1992) NMR studies of the conformational change in human N-p21ras produced by replacement of bound GDP with the GTP analog GTP gamma S. Biochemistry 31:10208-16
Pochapsky, T C; Ye, X M (1991) 1H NMR identification of a beta-sheet structure and description of folding topology in putidaredoxin. Biochemistry 30:3850-6
Hall, K B; McLaughlin, L W (1991) Thermodynamic and structural properties of pentamer DNA.DNA, RNA.RNA, and DNA.RNA duplexes of identical sequence. Biochemistry 30:10606-13
Lowry, D F; Cool, R H; Redfield, A G et al. (1991) NMR study of the phosphate-binding elements of Escherichia coli elongation factor Tu catalytic domain. Biochemistry 30:10872-7
Hall, K B; Sampson, J R (1990) Structural investigation of the in vitro transcript of the yeast tRNA(phe) precursor by NMR and nuclease mapping. Nucleic Acids Res 18:7041-7
Redfield, A G; Papastavros, M Z (1990) NMR study of the phosphoryl binding loop in purine nucleotide proteins: evidence for strong hydrogen bonding in human N-ras p21. Biochemistry 29:3509-14
Massefski Jr, W; Redfield, A G; Hare, D R et al. (1990) Molecular structure of charybdotoxin, a pore-directed inhibitor of potassium ion channels. Science 249:521-4

Showing the most recent 10 out of 24 publications