We want to relate the diversity of amino acid sequences among H1 histone subtypes and H1 variants (e.g. H1o and H1t) to the role of H1 in the structure of the chromosome. In particular we are interested in differences among H1 histones in their ability to condense chromatin (and related models) into higher order folding as a function of salt concentration. Complexes of individual kinds of H1 and chromatin fragments will be studied by circular dichroism, hydrodynamics, nuclease digestion and electron microscopy. Structural diversity among H1 subtypes and variants will be measured by amino acid sequencing and by immunological techniques. The kinetics and location of H1o will be studied throughout an extended period as cultured cells stop dividing and maintain themselves in a non-dividing state. When cells stop dividing, the non histones HMG1 and HMG2 are lost from the cell if the cells commit themselves to differentiation, and we want to look at the mechanism of this change in terms of proteolytic degradation or modification of the HMG's. These studies are aimed at showing if H1 histones play specific roles in controlling the dynamics of chromatin in cell division and differentiation, and so are related to problems of normal and abnormal growth.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM020338-19
Application #
3270000
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1978-06-01
Project End
1988-05-31
Budget Start
1986-06-01
Budget End
1987-05-31
Support Year
19
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of California Berkeley
Department
Type
Schools of Arts and Sciences
DUNS #
094878337
City
Berkeley
State
CA
Country
United States
Zip Code
94704