Abstract

The general goal of these investigations is to elucidate mechanisms of solute transport in the membranes of liver mitochondria and the relationships between these mechanisms and processes of cellular metabolism. Specifically some investigations are focusing on the energy-linked uptake of inorganic cations by mitochondria. The kinetics of unidirectional cation influx is being examined in relation to a proposed non-electrogenic transport mechanism. Some experiments are testing effects of inhibitors of oxidative phosphorylation in order to evaluate the extent to which the mechanism(s) of respiration-dependent cation influx may be directly linked to the mechanism of energy transduction. Other experiments are examinig the mechanism by which the urea cycle intermediate, ornithine, enters liver mitochondria.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM020726-09
Application #
3270134
Study Section
Physical Biochemistry Study Section (PB)
Project Start
1977-01-01
Project End
1986-12-31
Budget Start
1985-01-01
Budget End
1985-12-31
Support Year
9
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Rensselaer Polytechnic Institute
Department
Type
Schools of Arts and Sciences
DUNS #
002430742
City
Troy
State
NY
Country
United States
Zip Code
12180

  Publications

Paliwal, R; Costa, G; Diwan, J J (1992) Purification and patch clamp analysis of a 40-pS channel from rat liver mitochondria. Biochemistry 31:2223-9
Costa, G; Kinnally, K W; Diwan, J J (1991) Patch clamp analysis of a partially purified ion channel from rat liver mitochondria. Biochem Biophys Res Commun 175:305-10
Diwan, J J; Paliwal, R; Kaftan, E et al. (1990) A mitochondrial protein fraction catalyzing transport of the K+ analog T1+. FEBS Lett 273:215-8
Diwan, J J; Moore, C (1990) Effects of some protein-reactive compounds on K+ flux into mitochondria. Biochem Int 22:843-8
Diwan, J J; Haley, T; Sanadi, D R (1988) Reconstitution of transmembrane K+ transport with a 53 kilodalton mitochondrial protein. Biochem Biophys Res Commun 153:224-30
Diwan, J J; Yune, H H; Bawa, R et al. (1988) Enhanced uptake of spermidine and methylglyoxal-bis(guanylhydrazone) by rat liver mitochondria following outer membrane lysis. Biochem Pharmacol 37:957-61
Diwan, J J; Haley, T; Moore, C (1988) Sensitivity of mitochondrial Mg++ flux to reagents which affect K+ flux. J Bioenerg Biomembr 20:261-71
Diwan, J J (1986) Effect of quinine on mitochondrial K+ and Mg++ flux. Biochem Biophys Res Commun 135:830-6
Diwan, J J; Srivastava, J; Moore, C et al. (1986) Stimulation of K+ flux into mitochondria by phenylarsine oxide. J Bioenerg Biomembr 18:123-34
Diwan, J J (1985) Ba2+ uptake and the inhibition by Ba2+ of K+ flux into rat liver mitochondria. J Membr Biol 84:165-71