Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
3R01GM022526-11S1
Application #
3564429
Study Section
(MG)
Project Start
1978-09-01
Project End
1988-02-29
Budget Start
1987-12-01
Budget End
1988-02-29
Support Year
11
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Harvard University
Department
Type
DUNS #
071723621
City
Cambridge
State
MA
Country
United States
Zip Code
02138
Sigal, G B; Bamdad, C; Barberis, A et al. (1996) A self-assembled monolayer for the binding and study of histidine-tagged proteins by surface plasmon resonance. Anal Chem 68:490-7
Astromoff, A; Ptashne, M (1995) A variant of lambda repressor with an altered pattern of cooperative binding to DNA sites. Proc Natl Acad Sci U S A 92:8110-4
Ohashi, Y; Brickman, J M; Furman, E et al. (1994) Modulating the potency of an activator in a yeast in vitro transcription system. Mol Cell Biol 14:2731-9
Reece, R J; Rickles, R J; Ptashne, M (1993) Overproduction and single-step purification of GAL4 fusion proteins from Escherichia coli. Gene 126:105-7
Bushman, F D; Shang, C; Ptashne, M (1989) A single glutamic acid residue plays a key role in the transcriptional activation function of lambda repressor. Cell 58:1163-71
Bushman, F D; Ptashne, M (1988) Turning lambda Cro into a transcriptional activator. Cell 54:191-7
Irwin, N; Ptashne, M (1987) Mutants of the catabolite activator protein of Escherichia coli that are specifically deficient in the gene-activation function. Proc Natl Acad Sci U S A 84:8315-9
Ptashne, M (1986) Gene regulation by proteins acting nearby and at a distance. Nature 322:697-701
Bushman, F D; Ptashne, M (1986) Activation of transcription by the bacteriophage 434 repressor. Proc Natl Acad Sci U S A 83:9353-7
Hochschild, A; Douhan 3rd, J; Ptashne, M (1986) How lambda repressor and lambda Cro distinguish between OR1 and OR3. Cell 47:807-16

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