Abstract

The toxins from Conus geographus venom have been purified. These fall into four classes; two act on the peripheral neuromuscular system and two act on the central nervous system of vertebrates. The first class has been most well characterized; primary sequences of three homologous toxins of this class have been determined, and a preliminary secondary sequence assignment has been completed. These are all basic peptides of 13-15 amino acids, with two disulfide bonds and blocked carboxyl terminii. In the coming year, we plan to determine which structural determinants of the peptides are important for biological activity. Primary sequence determination of the other classes of toxins will also be carried out where feasible.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM022737-08
Application #
3271280
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1978-04-01
Project End
1987-12-31
Budget Start
1986-01-01
Budget End
1986-12-31
Support Year
8
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Utah
Department
Type
Schools of Arts and Sciences
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112

  Publications

Lev-Ram, V; Olivera, B M; Levitan, I B (1994) A toxin from the venom of the predator snail Conus textile modulates ionic currents in Aplysia bursting pacemaker neuron. Brain Res 640:48-55
Haack, J A; Parks, T N; Olivera, B M (1993) Conantokin-G antagonism of the NMDA receptor subtype expressed in cultured cerebellar granule cells. Neurosci Lett 163:63-6
Albensi, B C; Ryujin, K T; McIntosh, J M et al. (1993) Localization of [125I]omega-conotoxin GVIA binding in human hippocampus and cerebellum. Neuroreport 4:1331-4
Haack, J A; Kinser, P; Yoshikami, D et al. (1993) Biotinylated derivatives of omega-conotoxins GVIA and MVIID: probes for neuronal calcium channels. Neuropharmacology 32:1151-9
Santos, A D; Imperial, J S; Chaudhary, T et al. (1992) Heterodimeric structure of the spider toxin omega-agatoxin IA revealed by precursor analysis and mass spectrometry. J Biol Chem 267:20701-5
McIntosh, J M; Adams, M E; Olivera, B M et al. (1992) Autoradiographic localization of the binding of calcium channel antagonist, [125I]omega-agatoxin IIIA, in rat brain. Brain Res 594:109-14
Ramilo, C A; Zafaralla, G C; Nadasdi, L et al. (1992) Novel alpha- and omega-conotoxins from Conus striatus venom. Biochemistry 31:9919-26
Hillyard, D R; Monje, V D; Mintz, I M et al. (1992) A new Conus peptide ligand for mammalian presynaptic Ca2+ channels. Neuron 9:69-77
Colledge, C J; Hunsperger, J P; Imperial, J S et al. (1992) Precursor structure of omega-conotoxin GVIA determined from a cDNA clone. Toxicon 30:1111-6
Olivera, B M; Imperial, J S; Cruz, L J et al. (1991) Calcium channel-targeted polypeptide toxins. Ann N Y Acad Sci 635:114-22

Showing the most recent 10 out of 32 publications