The study is aimed at the analysis of the control of gene expression during the transition of proliferating precursor cells to postmitotic, terminally differentiated muscle cells. Myogenic cell lines, primary cultures, and cell lines derived from animals with hereditary diseases affecting the muscle tissue are being applied in this study. Recombinant plasmids containing cDNA sequences homologous to muscle-specific mRNA were constructed and applied as probes to follow gene expression at the RNA level and to screen rat genomic libraries for the corresponding genes. Recombinant phages containing DNA inserts homologous to myosin heavy chain, myosin light chain 2 and actin genes were isolated. Their structure and organization is being investigated.
| Einat, P; Shani, M; Yaffe, D (1990) The amount of the endogenous and exogenous skeletal muscle actin mRNA in the heart of transgenic mice is affected by the genotype of the cardiac actin gene. Differentiation 44:36-41 |
| Einat, P; Bergman, Y; Yaffe, D et al. (1987) Expression in transgenic mice of two genes of different tissue specificity integrated into a single chromosomal site. Genes Dev 1:1075-84 |
| Shani, M (1985) Tissue-specific expression of rat myosin light-chain 2 gene in transgenic mice. Nature 314:283-6 |
| Yaffe, D; Nudel, U; Mayer, Y et al. (1985) Highly conserved sequences in the 3' untranslated region of mRNAs coding for homologous proteins in distantly related species. Nucleic Acids Res 13:3723-37 |
| Nudel, U; Greenberg, D; Ordahl, C P et al. (1985) Developmentally regulated expression of a chicken muscle-specific gene in stably transfected rat myogenic cells. Proc Natl Acad Sci U S A 82:3106-9 |
