We are studying the organization and regulation of expression of the eukaryotic genetic loci that play a central role in the development of a tissue; the zein gene loci that function during endosperm development in Zea mays. The developing endosperm tissue of maize offers a favorable system since the synthesis of its storage proteins represents the one time expression of genes in which massive amounts of protein are synthesized in a short period of time. The objectives of the proposed research are to: 1. Clone the genome regions of maize that code for zein mRNA. 2. Examine the structural interrelationships among the zein genetic loci and their zein mRNAs. 3. Examine the nucleotide structure of the zein genetic loci by restriction enzyme analysis and nucleotide sequence analysis.
| Liu, C N; Rubenstein, I (1993) Transcriptional characterization of an alpha-zein gene cluster in maize. Plant Mol Biol 22:323-36 |
| Liu, C N; Rubenstein, I (1992) Molecular characterization of two types of 22 kilodalton alpha-zein genes in a gene cluster in maize. Mol Gen Genet 234:244-53 |
| Liu, C N; Rubenstein, I (1992) Genomic organization of an alpha-zein gene cluster in maize. Mol Gen Genet 231:304-12 |
| Chen, K Q; Rubenstein, I (1991) Characterization of the structure and transcription of an ubiquitin fusion gene from maize. Gene 107:205-12 |
| Veretnik, S; Rubenstein, I (1990) Nucleotide sequence of a maize U6 gene. Nucleic Acids Res 18:3661 |
| Larkin, J C; Hunsperger, J P; Culley, D et al. (1989) The organization and expression of a maize ribosomal protein gene family. Genes Dev 3:500-9 |
| Snustad, D P; Hunsperger, J P; Chereskin, B M et al. (1988) Maize glutamine synthetase cDNAs: isolation by direct genetic selection in Escherichia coli. Genetics 120:1111-23 |
