Abstract

The broad objective of this proposal is to understand how stringent amino acid control operates to regulate the expression of an appreciable fraction of E. coli genes. Using a selected ribosomal cistron promoter region, a sizeable degree of definition of promoter characteristics have been possible. It seems that a fairly thorough understanding of the structure and activity of the ribosomal promoter would be needed for further studies on its control.
The aim of this proposal is to correlate structure of the DNA of the rRNA with specific characteristics of the transcriptional control of these promoters. Further work will be identification of other factors than ppGpp participating in the control of these promoters and their mode of action. Recombinant plasmids and phages carrying the rRNA promoters fused to easy assayed genes will be used for isolation of both host and rRNA promoter mutants. Studies on the structure of rRNA operons of Mycoplasma will also be performed. The structure and regulation of the RelA gene will be studied for the understanding of cell growth control.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
2R01GM025286-07A1
Application #
3272905
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1978-08-01
Project End
1988-08-31
Budget Start
1985-09-05
Budget End
1986-08-31
Support Year
7
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Hebrew University of Jerusalem
Department
Type
DUNS #
600044978
City
Jerusalem
State
Country
Israel
Zip Code
91904

  Publications

Schreiber, G; Ron, E Z; Glaser, G (1995) ppGpp-mediated regulation of DNA replication and cell division in Escherichia coli. Curr Microbiol 30:27-32
Aviv, M; Giladi, H; Schreiber, G et al. (1994) Expression of the genes coding for the Escherichia coli integration host factor are controlled by growth phase, rpoS, ppGpp and by autoregulation. Mol Microbiol 14:1021-31
Gafny, R; Cohen, S; Nachaliel, N et al. (1994) Isolated P2 rRNA promoters of Escherichia coli are strong promoters that are subject to stringent control. J Mol Biol 243:152-6
Gropp, M; Eizenman, E; Glaser, G et al. (1994) A relA(S) suppressor mutant allele of Bacillus subtilis which maps to relA and responds only to carbon limitation. Gene 140:91-6
Gross, S R; Levine, P H; Metzger, S et al. (1989) Recombination and replication of plasmid-like derivatives of a short section of the mitochondrial chromosome of Neurospora crassa. Genetics 121:693-701
Hyman, H C; Gafny, R; Glaser, G et al. (1988) Promoter of the Mycoplasma pneumoniae rRNA operon. J Bacteriol 170:3262-8
Gafny, R; Hyman, H C; Razin, S et al. (1988) Promoters of Mycoplasma capricolum ribosomal RNA operons: identical activities but different regulation in homologous and heterologous cells. Nucleic Acids Res 16:61-76
Metzger, S; Dror, I B; Aizenman, E et al. (1988) The nucleotide sequence and characterization of the relA gene of Escherichia coli. J Biol Chem 263:15699-704
Hyman, H C; Gafny, R; Glaser, G et al. (1987) Transcription control elements of the Mycoplasma pneumoniae rRNA operon. Isr J Med Sci 23:585-90