Protein Synthesis in Prokaryotes and Eukaryotes

Wahba, Albert

Abstract

The overall objective of the proposed research is to further our understanding of the mechanism of protein biosynthesis, and study the role of the different factors required for the translation of messenger RNA. In addition, we are interested in determining which factors are responsible for the regulation of protein synthesis during encystment and development of Artemia embryos. In the following year our research aims include: a. An examination of the conformational changes of E. coli 30S and 50S ribosomal subunits following their interaction with chain initiation factor 3 and ribosomal protein S1. b. Both dormant and developing embryos of the brine shrimp Artemia contain two factors, Co-eIF-2(A) and Co-eIF-2(B), which stimulate eIF-2 activity. Direct complex formation between eIF-2 and these factors will be determined. The factors will also be examined for stimulating the release of eIF-2-GDP from the 40S ribosomal subunit and for recycling eIF-2. The function of these factors in relation to the activity of phosphorylated eIF-2 will be assayed. c. Isolation and characterization of chain initiation factor 3 from dormant and developing embryos of Artemia.

Funding Agency

Agency: National Institute of Health (NIH)
Institute: National Institute of General Medical Sciences (NIGMS)
Type: Research Project (R01)
Project #: 5R01GM025451-07
Application #: 3273032
Study Section: Molecular Biology Study Section (MBY)

Project Start: 1978-09-01
Project End: 1987-12-31
Budget Start: 1986-01-01
Budget End: 1986-12-31
Support Year: 7
Fiscal Year: 1986
Total Cost
Indirect Cost

Institution

Name: University of Mississippi Medical Center
Department
Type: Schools of Medicine
DUNS #: 928824473

City: Jackson
State: MS
Country: United States
Zip Code: 39216

Related projects


NIH 1992 R01 GM	Mechanisms & Regulation of Protein Synthesis Wahba, Albert J. / University of Mississippi Medical Center
NIH 1991 R01 GM	Mechanisms & Regulation of Protein Synthesis Wahba, Albert J. / University of Mississippi Medical Center
NIH 1990 R01 GM	Mechanisms & Regulation of Protein Synthesis Wahba, Albert J. / University of Mississippi Medical Center
NIH 1989 R01 GM	Mechanisms & Regulation of Protein Synthesis Wahba, Albert J. / University of Mississippi Medical Center
NIH 1988 R01 GM	Mechanisms & Regulation of Protein Synthesis Wahba, Albert J. / University of Mississippi Medical Center
NIH 1987 R01 GM	Protein Synthesis in Prokaryotes and Eukaryotes Wahba, Albert J. / University of Mississippi Medical Center
NIH 1986 R01 GM	Protein Synthesis in Prokaryotes and Eukaryotes Wahba, Albert J. / University of Mississippi Medical Center
NIH 1985 R01 GM	Protein Synthesis in Prokaryotes and Eukaryotes Wahba, Albert J. / University of Mississippi Medical Center