Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM026108-18
Application #
2174613
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1978-09-01
Project End
1999-03-31
Budget Start
1996-04-01
Budget End
1997-03-31
Support Year
18
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Virginia
Department
Biochemistry
Type
Schools of Medicine
DUNS #
001910777
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
Mesner, Larry D; Dijkwel, Pieter A; Hamlin, Joyce L (2015) Purification of restriction fragments containing replication intermediates from complex genomes for 2-D gel analysis. Methods Mol Biol 1300:261-77
Mesner, Larry D; Valsakumar, Veena; Cieslik, Marcin et al. (2013) Bubble-seq analysis of the human genome reveals distinct chromatin-mediated mechanisms for regulating early- and late-firing origins. Genome Res 23:1774-88
Wong, Philip G; Winter, Sherry L; Zaika, Elena et al. (2011) Cdc45 limits replicon usage from a low density of preRCs in mammalian cells. PLoS One 6:e17533
Mesner, Larry D; Valsakumar, Veena; Karnani, Neerja et al. (2011) Bubble-chip analysis of human origin distributions demonstrates on a genomic scale significant clustering into zones and significant association with transcription. Genome Res 21:377-89
Hamlin, Joyce L; Mesner, Larry D; Dijkwel, Pieter A (2010) A winding road to origin discovery. Chromosome Res 18:45-61
Mesner, Larry D; Hamlin, Joyce L (2009) Isolation of restriction fragments containing origins of replication from complex genomes. Methods Mol Biol 521:315-28
Mesner, Larry D; Dijkwel, Pieter A; Hamlin, Joyce L (2009) Purification of restriction fragments containing replication intermediates from complex genomes for 2-D gel analysis. Methods Mol Biol 521:121-37
Hamlin, J L; Mesner, L D; Lar, O et al. (2008) A revisionist replicon model for higher eukaryotic genomes. J Cell Biochem 105:321-9
Czajkowsky, Daniel M; Liu, Jie; Hamlin, Joyce L et al. (2008) DNA combing reveals intrinsic temporal disorder in the replication of yeast chromosome VI. J Mol Biol 375:12-9
Mesner, Larry D; Hamlin, Joyce L (2005) Specific signals at the 3' end of the DHFR gene define one boundary of the downstream origin of replication. Genes Dev 19:1053-66

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