The MyoD cDNA, when expressed under the control of a viral LTR and transfected into a variety of fibroblast and adipoblast cell lines, converts these cells to muscle. While not yet proven MyoD acts as if it were a master regulatory gene for myogenesis. The MyoD protein is nuclear and preliminary work suggests it binds DNA. The present proposal is to: (1) Characterize MyoD using detailed mutagenic analysis. (2) Study its binding to specific DNA sequences; study its interaction with other cellular proteins. (3) Explore preliminary indications that MyoD positively activates its own synthesis but negatively activates its mRNA utilization.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM026176-14
Application #
3273676
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1979-05-01
Project End
1994-04-30
Budget Start
1992-05-01
Budget End
1993-04-30
Support Year
14
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Fred Hutchinson Cancer Research Center
Department
Type
DUNS #
075524595
City
Seattle
State
WA
Country
United States
Zip Code
98109