We propose to continue our studies of the mechanism of transcriptional control in the mammalian cell relying heavily on adenovirus gene expression as a system to study. The major thrust of the work is to understand the manner in which the adenovirus E1A gene product effects transcriptional activation of viral genes and how this reflects similar processes in the uninfected cell. To accomplish this, we will focus on two major aspects of the problem: sequences essential for transcription and regulation and proteins that interact with these sequences. First, we will further define sequences in certain inducible promoters that are essential for the function of the gene. These include the adenovirus E1A enhancer element that are essential for its function. With this information in hand, we will proceed to define proteins that interact with these sequences and thus are presumably participating in the process of transcriptional control. Finally, we will isolate additional cellular genes that are subject to E1A Control.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Project (R01)
Project #
5R01GM026765-08
Application #
3274215
Study Section
Molecular Biology Study Section (MBY)
Project Start
1979-07-01
Project End
1987-05-31
Budget Start
1986-09-01
Budget End
1987-05-31
Support Year
8
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Rockefeller University
Department
Type
Graduate Schools
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065
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Raychaudhuri, P; Bagchi, S; Neill, S D et al. (1990) Activation of the E2F transcription factor in adenovirus-infected cells involves E1A-dependent stimulation of DNA-binding activity and induction of cooperative binding mediated by an E4 gene product. J Virol 64:2702-10
Chellappan, S P; Nevins, J R (1990) DNA octamer element can confer E1A trans-activation, and adenovirus infection results in a stimulation of the DNA-binding activity of OTF-1/NFIII factor. Proc Natl Acad Sci U S A 87:5878-82
Bagchi, S; Raychaudhuri, P; Nevins, J R (1990) Adenovirus E1A proteins can dissociate heteromeric complexes involving the E2F transcription factor: a novel mechanism for E1A trans-activation. Cell 62:659-69

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